合胞素通过促进线粒体去极化诱导小鼠淋巴瘤细胞EL4细胞凋亡  

Syncytin induces apoptosis in mouse lymphoma cell line EL4 through promoting mitochondrial depolarization

作　　者：孙鹥[1] 范欣[1] 徐亚 井敏敏 Sun Yi;Fan Xin;Xu Ya;Jing Minmin(Department of Clinical Laboratories,The First People’s Hospital of Yunnan Province,Kunming 650032,China)

机构地区：[1]云南省第一人民医院检验科,昆明650032

出　　处：《中国组织化学与细胞化学杂志》2018年第5期405-415,共11页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金(81100377);云南省应用基础研究(昆医联合专项2013FB203);云南省医学学科带头人培养项目(D-201670)

摘　　要：目的探讨人类内源性病毒W家族囊膜蛋白合胞素(syncytin)对白血病细胞凋亡的影响及其信号机制。方法对小鼠淋巴瘤细胞EL4细胞稳转表达合胞素,显微镜观察细胞形态变化,流式细胞术检测细胞凋亡、细胞周期及线粒体膜电位情况,用试剂盒检测PKC和Caspase-3活性。结果合胞素稳定过表达后,EL4细胞形态和PKC活性无明显改变,细胞凋亡明显增加,线粒体膜电位去极化增加,Caspase-3活性增强。结论合胞素可诱导小鼠淋巴瘤细胞EL4细胞线粒体途径细胞凋亡,在白血病的发生发展过程中发挥重要作用。Objective To explore the effect of syncytin,the envelop protein of the human endogenous virus W family,on the apoptosis of leukemia cells and its signaling mechanisms.Methods The mouse lymphoma cell line EL4 was transfected to stablely express syncytin,then the changes of cell morphology were observed with microscope;cell apoptosis,cell cycle and mitochondrial membrane potential were detected by flow cytometry;PKC and Caspase activities were detected by kit.Results After stable overexpression of syncytin,while no significant changes in the cell morphology of EL4 and the activity of PKC,there was a significant increase in the cell apoptosis,the depolarization of mitochondrial membrane potential and the activity of Caspase-3.Conclusion Syncytin may induce cell apoptosis via mitochondrial signal pathway in mouse lymphoma cell line EL4 and play an important role in the genesis and progression of leukemia.

关 键 词：人内源性逆转录病毒 合胞素 白血病 凋亡 线粒体 

分 类 号：R733.7[医药卫生—肿瘤]