趋化因子受体7在乳腺癌组织中的表达及其与血管内皮生长因子-D的关系  

Expression of CCR7 in breast carcinoma and its relationship with vascular endothelial growth factor-D

作　　者：赵云霞[1] 姜丽娜 赵瑾[1] Zhao Yunxia;Jiang Lina;Zhao Jin(Department of Pathophysiology,Bengbu Medical College,Bengbu 233030,China;Key Laboratory of Infection and Immunology of Anhui Province,Bengbu 233030,China)

机构地区：[1]蚌埠医学院病理生理学教研室 [2]安徽省感染与免疫重点实验室,蚌埠233030

出　　处：《中国组织化学与细胞化学杂志》2018年第5期440-445,464,共7页Chinese Journal of Histochemistry and Cytochemistry

基　　金：安徽省高等学校自然科学研究重点项目(KJ2016A867);安徽省高等学校自然科学研究一般项目(KJ2015b106by);蚌埠医学院科技发展基金项目(BYKF1708)

摘　　要：目的探讨趋化因子受体7(chemokine receptor 7, CCR7)在乳腺癌组织中的表达及与血管内皮生长因子-D(vascular endothelial growth factor-D, VEGF-D)在乳腺癌淋巴转移中的关系。方法应用免疫组织化学Elivision法和原位杂交方法检测98例乳腺癌组织及30例乳腺正常组织中CCR7、VEGF-D及其mRNA表达情况,D2-40标记微淋巴管,计数微淋巴管密度(lymphatic vessel density, LVD)。结果乳腺癌组织中CCR7和其mRNA的表达阳性率分别为67.3%和71.4%,显著高于对照组;VEGF-D和其mRNA的表达阳性率分别为69.4%和73.5%,也显著高于对照组。CCR7水平在乳腺癌淋巴结转移阳性组显著增高;CCR7及VEGF-D表达阳性组LVD值分别为12.91±3.38和13.38±3.81,均显著高于CCR7及VEGF-D表达阴性组。CCR7表达阳性组中VEGF-D表达显著高于CCR7表达阴性组,CCR7表达与VEGF-D表达呈正相关。结论 CCR7通过VEGF-D诱导淋巴管新生,是乳腺癌淋巴转移的重要因子。Objective To investigate CCR7(chemokine receptor 7,CCR7)expression in breast carcinoma and its relationship with VEGF-D(vascular endothelial growth factor-D,VEGF-D)in lymphatic metastasis of breast carcinoma.Methods In 98 cases with breast carcinoma and 30 normal breast tissues,the protein levels of CCR7 and VEGF-D and their mRNAs were examined by immunohistochemical Elivision method and in situ hybridization,respectively.In addition to the above tissues,lymphatic microvessels were marked by D2-40 and lymphatic microvessel density(LVD)was counted.Results In breast carcinoma,the positive rates of CCR7 protein and mRNA were 67.3%and 71.4%respectively,significantly higher than those of controls.The positive rates of VEGF-D protein and mRNA were 69.4%and 73.5%respectively,significantly higher than those of controls.The level of CCR7 was significantly increased in the group of breast carcinoma with lymphatic metastasis.The LVD values of cases with positive expression of CCR7 and VEGF-D protein were 12.91±3.38 and 13.38±3.81 respectively,which were significantly higher than those of cases with negative expression.VEGF-D protein expression was higher in cases with CCR7 protein positive expression than in cases with CCR7 protein negative expression,and there was positive correlation between CCR7 protein expression and VEGF-D protein expression.Conclusion CCR7 induces lymphangiogenesis through VEGF-D,which is an important factor in lymphatic metastasis of breast carcinoma.

关 键 词：乳腺肿瘤 趋化因子受体7 血管内皮生长因子-D 淋巴转移 

分 类 号：R737.9[医药卫生—肿瘤]