基于脂质代谢组学研究重离子束辐射诱导的酿酒酵母线粒体损伤  

Lipidomics Studies on Mitochondrial Damage of Saccharomyces Cerevisiae Induced by Heavy Ion Beam Radiation

作　　者：张苗苗[1,2,3] 郭晓鹏 刘瑞媛[1] 马良 高越[1,2] 陆栋[1,3] 李文建 ZHANG Miao-Miao;GUO Xiao-Peng;LIU Rui-Yuan;MA Liang;GAO Yue;LU Dong;LI Wen-Jian(Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou 73000,China;University of Chinese Academy of Sciences,Beijing 100049,China;Gansu Provincial Key Laboratory of Microbial Resources Exploition and Application,Lanzhou 73000,China)

机构地区：[1]中国科学院近代物理研究所,兰州730000 [2]中国科学院大学,北京100049 [3]甘肃省微生物资源开发利用重点实验室,兰州73000

出　　处：《分析化学》2018年第11期1714-1723,共10页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.11575259);中国科学院重点部署项目(No.KFZD-SW-109)资助~~

摘　　要：采用超高效液相色谱-电喷雾离子化-质谱(UHPLC-ESI-MS)技术,结合多元变量及单变量的数据分析方法,研究了重离子束辐射后线粒体受损的酿酒酵母(Res D-4)的脂质代谢组学。检测了对照组(Control)及Res D-4的脂质代谢物组成,共鉴定出648种脂质分子。研究结果表明,正交偏最小二乘判别分析(OPLSDA)能准确区分Res D-4与Control的脂质代谢物;通过变量权重值(VIP)、变异倍数(FC)、差异性(p)分析相结合,最终筛选出甘油二酯(DG)(16∶0/18∶1)、溶血磷脂酰胆碱(LPC)(18∶0)、溶血磷脂乙醇胺(LPE)(16∶0)、溶血磷脂酰肌醇(LPI)(18∶0)、溶血磷脂酰丝氨酸(LPS)(18∶0)、磷脂酸(PA)(16∶0/18∶0)、磷脂酰胆碱(PC)(31∶0)、磷脂酰乙醇胺(PE)(16∶0/10∶0)、磷脂酰肌醇(PI)(15∶0/18∶0)、磷脂酰丝氨酸(PS)(16∶0/16∶0)、硫代异鼠李糖甘油二酯(SQDG)(37:4)和硫代异鼠李糖甘油二酯(SQDG)(15∶0/16∶0/18∶0)共12个亚类、54种显著性差异脂质分子;显著性差异脂质分子表达量分析显示,Res D-4中DG、TG、SQDG、PA、PC、PI、LPC、LPE、LPI的表达量上调,PE、PS、LPS的表达量下调。根据上述实验结果,推测酿酒酵母可能通过调节脂质成分的含量来应对重离子束辐射引起的线粒体损伤,显著性差异脂质分子可能是重离子束辐射诱导酿酒酵母线粒体损伤后的潜在标志物。Ultra high performance liquid chromatography-electrospray ionization-mass spectrometry(UHPLC-ESI-MS)combined with multivariate and univariate data analysis methods was used to analyze lipid metabolomics of mitochondria-damaged Saccharomyces cerevisiae(ResD-4)after heavy ion beam radiation.The composition of lipid metabolites in control and ResD-4 was detected,and a total of 648 lipid molecules were identified.The results showed that the orthogonal partial least squares discriminant analysis(OPLS-DA)could accurately distinguish the lipid metabolites of ResD-4 and control.Based on variable importance for the projection(VIP),variation multiple(FC)and difference analysis(p),12 subfamilies,54 types of significant difference in lipid molecules were finally screened including diacylglycerol(DG)(16∶0/18∶1),lysophosphatidylcholine(LPC)(18∶1),lysophosphatidylethanolamine(LPE)(16∶0),lysophosphatidylinositol(LPI)(18∶1),lysophosphatidylserine(LPS)(18∶1),phosphatidic acid(PA)(16∶0/18∶1),phosphatidylcholine(PC)(31∶1),phosphatidylethanolamine(PE)(16∶0/10∶0),phosphatidylinositol(PI)(15∶0/18∶1),phosphatidylserine(PS)(16∶0/16∶1),sulfoquinovosyl diacylglycerol(SQDG)(37∶4),triglyceride(TG)(15∶0/16∶0/18∶1),etc.Significant differences in the expression level of lipid molecules showed that the expression levels of DG,TG,SQDG,PA,PC,PI,LPC,LPE and LPI were up-regulated,and PE,PS and LPS were down-regulated.The results showed that S.cerevisiae might respond by adjusting the content of lipid components after mitochondrial damage caused by heavy ion beam radiation.The lipid molecules with significant difference expression level may be potential markers for S.cerevisiae mitochondrial damage induced by heavy ion beam radiation.

关 键 词：脂质组学 重离子 线粒体损伤 酿酒酵母 超高效液相色谱-电喷雾离子化-质谱 

分 类 号：O657.63[理学—分析化学] TS261.11[理学—化学]