明目消朦预处理对H_2O_2诱导人视网膜色素上皮细胞凋亡及氧化应激损伤的影响  

Effects of Mingmu Xiaomeng on hydrogen peroxide-induced apoptosis and oxidative stress injury in human retinal pigment epithelia

作　　者：袁灵梅[1] 贾洪亮[1] 王燕[2] 袁远[2] 尚亚南 曾志奎 邱波[2] YUAN Lingmei;JIA Hongliang;WANG Yan;YUAN Yuan;SHANG Yanan;ZENG Zhikui;QIU Bo(The Affiliated Hospital of Jiangxi University of Chinese Medicine,Nanchang 330006,China)

机构地区：[1]江西中医药大学附属医院,南昌330006 [2]广东省中医院 [3]解放军第一五二中心医院

出　　处：《山东医药》2018年第39期21-24,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(8150150299);广东省科学技术厅基金项目(2014A020221051)

摘　　要：目的观察明目消朦(MMXM)预处理对H_2O_2诱导人视网膜色素上皮细胞(RPE细胞)凋亡和氧化应激损伤的影响。方法取对数生长的RPE细胞,将细胞分为4组,A组加入含MMXM的无血清培养基,MMXM的终浓度为0. 5 mg/m L,24 h后加入200μmol/m L的H_2O_2(以制备氧化应激损伤模型); B组加入无血清的基础培养基,24 h后加入200μmol/m L的H_2O_2; C组加入含MMXM的无血清培养基,MMXM的终浓度为0. 5 mg/m L; D组加入无血清的基础培养基。比较各组细胞调亡率及ROS、MDA、SOD、GSH-PX。结果与B组比较,其余3组细胞凋亡率降低(P均<0. 05);与C、D组比较,A、B组细胞凋亡率升高(P均<0. 05)。与D组比较,A、B组ROS(+)、MDA升高,ROS(-)、SOD、GSH-PX降低(P均<0. 05);与B组比较,其余3组ROS(-)、SOD、GSH-PX升高,ROS(+)、MDA降低(P均<0. 05)。结论 MMXM预处理能有效减少H_2O_2诱导的RPE细胞凋亡,并可预防细胞氧化损伤。Objective To observe the effects of pretreatment of Mingmu Xiaomeng(MMXM)on H 2O 2-induced apoptosis and oxidative stress injury in human retinal pigment epithelial cells(RPE cells).Methods RPE cells in the logarithmic growth were divided into 4 groups:groups A,B,C,and D.Cells in the group A were added with serum-free medium containing 0.5 mg/mL MMXM,and 200μmol/mL H 2O 2 was added after 24 h(to prepare an oxidative stress damage model);serum-free basal medium was added to the cells in the group B,and 200μmol/mL H 2O 2 was added after 24 h;cells in the group C were added with serum-free medium containing MMXM with the final concentration of 0.5 mg/mL;serum-free basal medium was added to the cells in the group D.The apoptosis rate and reactive oxygen species(ROS),malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione peroxidase(GSH-PX)were analyzed.Results Group B had a higher apoptosis rate than the other three groups(P<0.05);compared with group C and D,the apoptosis rate increased in the group A and group B(P<0.05);compared with group D,ROS(+)and MDA increased,and ROS(-),SOD,and GSH-PX decreased in the group A and group B(all P<0.05);compared with group B,ROS(-),SOD,and GSH-PX increased and ROS(+)and MDA decreased in the other three groups(all P<0.05).Conclusion MMXM pretreatment can effectively reduce H 2O 2-induced apoptosis and prevent oxidative damage in RPE cells.

关 键 词：明目消朦 糖尿病视网膜病变 人视网膜色素上皮细胞 细胞凋亡 氧化应激损伤 

分 类 号：R774.1[医药卫生—眼科]