长链非编码RNA结肠癌相关转录本2在胃癌血清中的表达及临床意义  

Expression of long non-coding RNA CCAT2 in gastric cancer serum and its clinical significance

作　　者：孙晓杰[1] 郭红艳[2] 吴琦[1] 齐晓丹[1] 徐晶[2] 黄常志 张春晶[2] Sun Xiaojie;Guo Hongyan;Wu Qi;Qi Xiaodan;Xu Jing;Huang Changzhi;Zhang Chunjing(Department of Clinical Biochemistry,Qiqihar Medical College,Qiqihar 161006;Department of Biochemistry,Qiqihar Medical College,Qiqihar 161006;National Cancer Center/State Key Laboratory of Molecular Oncology,Cancer Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100021,China)

机构地区：[1]齐齐哈尔医学院临床生化教研室,齐齐哈尔161006 [2]齐齐哈尔医学院生物化学教研室,齐齐哈尔161006 [3]国家癌症中心/中国医学科学院北京协和医学院肿瘤医院分子肿瘤学国家重点实验室,北京100021

出　　处：《解剖学杂志》2018年第5期525-529,539,共6页Chinese Journal of Anatomy

基　　金：国家自然科学基金面上项目(81472208);黑龙江省留学归国科学基金项目(LC2011C34);齐齐哈尔医学院科学研究项目(QY2016M-05)

摘　　要：目的:检测长链非编码RNA结肠癌相关转录本2(CCAT2)在胃癌患者和正常对照组血清中的差异表达,探讨其对胃癌临床诊断的效能及意义。方法:通过实时荧光定量PCR方法检测胃癌和正常对照组血清CCAT2的表达,分析其与胃癌临床病理特征的相关性;通过受试者工作特征(ROC)曲线和危险分数分析法评估CCAT2对胃癌的诊断效能,并与肿瘤标志物癌胚抗原(CEA)、糖类抗原19-9(CA19-9)、糖类抗原72-4(CA72-4)以及甲胎蛋白(AFP)的诊断效能进行比较。结果:胃癌患者血清中CCAT2的表达量明显高于正常对照组;其表达水平与年龄、性别、肿瘤临床分期、淋巴结转移以及肿瘤远处转移等均不相关,但与肿瘤细胞的分化程度呈负相关。CCAT2诊断胃癌的曲线下面积为0.619,敏感度和特异度分别为78.63%和53.00%,阳性预测值和阴性预测值分别为66.18%和67.95%。与CA19-9、CEA以及CA72-4相比,诊断的敏感度显著增高。结论:CCAT2在胃癌血清中高表达,有可能成为胃癌临床诊断的潜在生物标志物。Objective:To detect the differential expression of long non-coding RNA colon cancer associated transcript 2(CCAT2)in gastric cancer patients and healthy controls,and to explore its efficacy and significance in the clinical diagnosis of gastric cancer.Methods:The expression of CCAT2 in gastric cancer and healthy controls was detected by real-time fluorescence quantitative PCR.The correlation between the expression of CCAT2 and the clinicopathological characteristics of gastric cancer was also analyzed.The diagnostic efficacy of CCAT2 in gastric cancer was evaluated by applying receiver operating characteristic(ROC)curve and risk score analysis,and was compared with that of tumor markers carcinoembryonic antigen(CEA),carbohydrate antigen 19-9(CA19-9),carbohydrate antigen 72-4(CA72-4),and alpha-fetoprotein(AFP).Results:The expression of CCAT2 in the serum of gastric cancer patients was significantly higher than that of the healthy controls,and the expression level was not related to age,gender,clinical stage,lymph node metastasis and distant metastasis of tumor,but it was negatively correlated with the degree of differentiation of tumor cells.The area under the curve of CCAT2 for diagnosis of gastric cancer was 0.619,and the sensitivity and specificity were 78.63%and 53.00%respectively.The positive predictive value and negative predictive value were 66.18%and 67.95%respectively.Compared with CA19-9,CEA and CA72-4,the sensitivity of diagnosis increased significantly.Conclusion:CCAT2 is highly expressed in gastric cancer serum and may be a potential biomarker for clinical diagnosis of gastric cancer.

关 键 词：长链非编码RNA 结肠癌相关转录本2 胃癌 受试者工作特征曲线 生物标志物 

分 类 号：R735.2[医药卫生—肿瘤]