采用ICP-MS/MS准确测定食品中钙和氯  

Accurate Determination of Calcium and Chlorine in Food with Inductively Coupled Plasma Tandem Mass Spectrometry

作　　者：周学忠 刘宏伟 ZHOU Xue-zhong;LIU Hong-wei(College of Material and Chemical Engineering,Hunan Institute of Technology,Hengyang 421002,China)

机构地区：[1]湖南工学院材料与化学工程学院,湖南衡阳421002

出　　处：《光谱学与光谱分析》2018年第11期3567-3571,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金面上项目(81603400);湖南省教育厅重点科研项目(14A035);湖南省重点学科建设项目(湘教发[2011]76号)资助

摘　　要：建立利用电感耦合等离子体串联质谱测定食品中钙和氯的分析方法。针对Ca的高丰度同位素^(40)Ca受到来自等离子气^(40)Ar的干扰,35 Cl受到来自^(16)O^(18) OH的严重干扰,在MS/MS模式下,利用碰撞/反应池技术,以H_2为反应气,使^(40)Ar^+与H_2发生反应,而^(40)Ca^+不与H_2发生反应,利用H_2原位质量法消除^(40)Ar^+对^(40)Ca^+的干扰;而^(35)Cl^+则能与H_2发生质量转移反应生成H2^(35)Cl^+,通过测定H_2^(35)Cl^+消除^(16)O^(18) OH^+对^(35)Cl^+的干扰。Ca和Cl在0.0～100.0μg·L^(-1)的浓度范围内线性关系良好,线性相关系数(R^2)≥0.9999,Ca和Cl的检出限分别为0.061和2.32μg·L^(-1)。采用系列国家标准物质验证了对方法的准确性和精密度,其测定结果与标准参考物质的认定值基本一致,表明方法的准确性好、精密度高。所建立的新方法可实现食品中钙和氯的准确测定。An analytical method for the determination of Ca and Cl elements in food was est ablished with inductively coupled plasma tandem mass spectrometry(ICP-MS/MS).T he most abundant isotope 40 Ca of Ca is suffering from intense interfer ences from the 40 Ar among the argon plasma gas,and 35 Cl is subjected to interference from 16 O 18 OH.To avoid the interfe rence,in the MS/MS mode,collision/reaction cell(CRC)was adopted using H 2 as the reaction gas,so that H 2 will reach with 40 Ar+inste ad of 40 Ca+,A H 2 on-mass method was used to eliminate the inter ference of 40 Ar+on 40 Ca+.As mass shift reaction could take place between 35 Cl+and H 2 to generate H 2 35 Cl+,thus the interference of 16 O 18 OH+on 35 Cl+can be eliminated by determining H 2 35 Cl+.Ca and Cl showed good linea rity in the concentration range of 0.0~100.0μg·L-1 with linear corr elation coefficient(R 2)≥0.999 9,and the detection limits of Ca and Cl were 0.061 and 2.32μg·L-1,respectively.The accuracy and precisi on of this method were verified by series of national standard materials.The r esults of this method were basically consistent with the certified values of the reference materials,indicating that the method of good accuracy and high prec ision.The new method can be used to achieve the accurate determination of Ca a nd Cl in food.

关 键 词：食品 电感耦合等离子体串联质谱 钙 氯 H 2反应 

分 类 号：O657.3[理学—分析化学]