miR-1253对肺腺癌细胞增殖与迁移侵袭能力的影响  

Effects of miR-1253 on proliferation,migration and invasion of lung adenocarcinoma cells

作　　者：张超[1] 牛意 张洁[2] 姚学敏[1] 高鹏 刘美月 魏晓梅 杨朝 刘春玲 张月恒[1] 李宏民 孙国贵[1] ZHANG Chao;NIU Yi;ZHANG Jie;YAO Xuemin;GAO Peng;LIU Meiyue;WEI Xiaomei;YANG Zhao;LIU Chunling;ZHANG Yueheng;LI Hongmin;SUN Guogui(Department of Radiation Oncology,Tangshan People s Hospital,Tangshan 063000,China)

机构地区：[1]唐山市人民医院放射肿瘤科,河北唐山063000 [2]唐山市人民医院病理科,063000

出　　处：《临床肿瘤学杂志》2018年第10期865-869,共5页Chinese Clinical Oncology

基　　金：国家自然科学基金资助项目(81402534);河北省自然科学基金资助项目(H2015105095);河北省青年拔尖人才支持项目(冀字[2016]10号);河北省"三三三人才工程"支持项目(A2016002090);河北省医学科学研究重点课题计划(ZD20140084);唐山市分子肿瘤研究与临床转化应用创新团队(15130234a)

摘　　要：目的探讨微小RNA-1253 (miR-1253)在肺腺癌细胞株中的表达及其对肺腺癌细胞增殖、迁移侵袭能力的影响。方法采用实时荧光定量PCR(QPCR)检测肺腺癌A549、NCI-H1299、NCI-H157、A973及GLC-82细胞株中的miR-1253表达水平,将miR-1253 mimics和miR-1253 inhibitor分别转染至A973和NCI-H157细胞,以转染阴性对照质粒NC的细胞为阴性对照(NC)组。MTS实验、克隆形成实验及Transwell迁移侵袭实验检测不同miR-1253表达对A973和NCI-H157细胞增殖、克隆形成及侵袭转移能力的影响。结果 QPCR检测结果显示,A549、NCI-H1299、NCI-H157、A973及GLC-82细胞中miR-1253相对表达量分别为0. 92±0. 06、0. 06±0. 03、1. 10±0. 26、0. 03±0. 01、0. 45±0. 08。A973细胞转染miR-1253 mimics后miR-1253相对表达量显著升高(P<0. 05),NCI-H157细胞转染miR-1253 inhibitor后miR-1253相对表达量显著下降(P<0. 05)。与NC组比较,转染miR-1253 mimics能够显著抑制肺腺癌细胞A973的增殖活性、平板克隆形成能力(166. 0±29. 3 vs. 371. 0±31. 4,P=0. 001)、迁移(91. 1±32. 1 vs. 166. 7±33. 9,P=0. 008)以及侵袭能力(74. 4±20. 5 vs. 145. 6±28. 8,P=0. 001);而miR-1253 inhibitor能够上调NCI-H157的增殖、平板克隆形成细胞数目(545. 0±61. 9 vs. 337. 0±39. 7,P=0. 008)、迁移(246. 7±36. 7vs. 151. 1±32. 9,P<0. 001)以及侵袭能力(231. 1±38. 8 vs. 137. 8±27. 3,P=0. 001)。结论 miR-1253可以抑制肺腺癌细胞的增殖与侵袭转移,可能作为肺腺癌基因治疗的有效靶点。Objective To investigate the expression of microRNA-1253(miR-1253)in lung adenocarcinoma cell lines and its effect on the proliferation,migration and invasion of lung adenocarcinoma cells.Methods Real-time fluorescence quantitative PCR(QPCR)was used to detect the expression of miR-1253 in lung adenocarcinoma cell lines A549,NCI-H1299,NCI-H157,A973 and GLC-82.miR-1253 mimics and miR-1253 inhibitor were transfected into A973 and NCI-H157 cells respectively.The cells transfected with negative control plasmid NC were used as negative control(NC)group.The effects of different expression of miR-1253 on proliferation,clone formation,invasion and migration of A973 and NCI-H157 cells were examined by cell proliferation assay,clone formation assay and Transwell migration and invasion assay.Results The results of QPCR showed that the relative expression of miR-1253 in A549,NCI-H1299,NCI-H157,A973 and GLC-82 cells were 0.92±0.06,0.06±0.03,1.10±0.26,0.03±0.01 and 0.45±0.08,respectively.After transfection of miR-1253 mimics into A973 cells,the relative expression of miR-1253 increased significantly(P<0.05),and decreased significantly after transfection of miR-1253 inhibitor into NCI-H157 cells(P<0.05).Compared with the NC group,the transfection of miR-1253 mimics could significantly inhibit the proliferation activity,the ability of plate cloning(166.0±29.3 vs.371.0±31.4,P=0.001)and the ability of migration(91.1±32.1 vs.166.7±33.9,P=0.008)and invasion(74.4±20.5 vs.145.6±28.8,P=0.001)of lung adenocarcinoma cell line A973;miR-1253 inhibitor can up-regulate the proliferation,number of plate cloning cells(545.0±61.9 vs.337.0±39.7,P=0.008),migration(246.7±36.7 vs.151.1±32.9,P<0.001)and invasion(231.1±38.8 vs.137.8±27.3,P=0.001)of NCI-H157.Conclusion miR-1253 could reduce malignant phenotype of lung adenocarcinoma cells,and therefore it can be used as an effective target for gene therapy of lung adenocarcinoma.

关 键 词：肺腺癌 微小RNA 侵袭转移 基因治疗 

分 类 号：R734.2[医药卫生—肿瘤]