肺腺癌组织精氨酸酶-2的表达及临床意义  

Expression and clinical significance of arginase-2 in lung adenocarcinoma

作　　者：肖锋[1] 徐光寰 顾春燕[1] 邵建国[3] 陈丽燕[1] 孙艳[1] 肖静文[1] XIAO Feng;XU Guang-huan;GU Chun-yan;SHAO Jian-guo;CHEN Li-yan;SUN Yan;XIAO Jing-wen(Department of Pathology,Nantong Third Hospital,Nantong University,Nantong 226006,Jiangsu,China;Department of General Surgery,Changhai Hospital,Navy Military Medical University,Shanghai 200433,China;Department of Gastroenterology,Nantong Third Hospital,Nantong University,Nantong 226006,Jiangsu,China)

机构地区：[1]南通大学附属南通第三医院病理科,南通226006 [2]海军军医大学附属长海医院普通外科,上海200433 [3]南通大学附属南通第三医院消化内科,南通226006

出　　处：《中国临床医学》2018年第5期743-747,共5页Chinese Journal of Clinical Medicine

基　　金：南通市市级科技计划项目(MS22015087;GJZ16007);南通市卫计委青年科研基金资助项目(WQ2015034;WQ2016016)~~

摘　　要：目的:探讨精氨酸酶-2(arginase-2,Arg-2)在肺腺癌中的表达及其临床病理学意义。方法:应用反转录-聚合酶链式反应(reverse transcriptase-polymerase chain reaction,RT-PCR)和蛋白质印迹法分别检测7例新鲜肺腺癌及对应癌旁肺组织以及1例正常肺组织中Arg-2mRNA和蛋白的表达情况;免疫组织化学法检测108例肺腺癌患者手术切除石蜡标本中Arg-2蛋白的表达及细胞定位。分析Arg-2表达与肺腺癌临床病理指标及预后的相关性。结果:肺腺癌组织中Arg-2mRNA和蛋白表达水平均明显高于癌旁肺组织和正常肺组织。Arg-2蛋白表达定位于细胞质。108例肺腺癌组织中Arg-2蛋白低表达74例,高表达34例;肺腺癌组织中Arg-2蛋白的表达水平明显高于癌旁肺组织(P<0.000 1)。Arg-2高表达与肺腺癌分化程度(P=0.000 6)、淋巴结转移(P=0.002 8)、TNM分期(P=0.021 5)及Ki-67细胞增殖指数(P<0.000 1)正相关。Arg-2高表达组患者的总生存率明显低于Arg-2低表达组(χ~2=6.762,P=0.009)。多因素Cox比例风险模型分析显示,Arg-2高表达不是肺腺癌的独立预后因素(P=0.051)。结论:Arg-2表达与肺腺癌增殖、分化、淋巴结转移及TNM分期相关;Arg-2高表达提示肺腺癌患者预后差,但不是独立预后因素。Objective:To investigate the expression of arginase-2(Arg-2)in lung adenocarcinoma and its clinicopathological significance.Methods:Reverse transcription-polymerase chain reaction(RT-PCR)and Western blotting were respectively used to detect the expressions of Arg-2 mRNA and protein in 7 cases of lung adenocarcinoma and paracancerous lung tissues,and 1 case of normal lung tissues.The immunohistochemical method was used to detect the expression and location of the Arg-2 protein in the paraffin specimens of 108 patients with lung adenocarcinoma.The correlation of Arg-2 expression with the clinicopathological indexes of lung adenocarcinoma and the prognosis of patients were analyzed.Results:Arg-2 mRNA and protein expression levels in lung adenocarcinoma tissues were significantly higher than those in adjacent and normal lung tissues.Immunohistochemistry showed that the positive signal of Arg-2 protein was located in the cytoplasm.In 108 cases of lung adenocarcinoma,74 cases had low expression of the Arg-2 protein,other 34 cases had high expression.The positive expression rate of Arg-2 in lung adenocarcinoma was higher than that in adjacent tissues(P<0.000 1).The high expression of Arg-2 was positively correlated with the differentiation degree of lung adenocarcinoma(P=0.000 6),lymph node metastasis(P=0.002 8),TNM stage(P=0.021 5),and Ki-67 cell proliferation index(P<0.000 1).The overall survival rate of Arg-2 high expression group was significantly lower than that of Arg-2 low expression group(χ2=6.762,P=0.009).Multivariate Cox proportional hazards model analysis showed that Arg-2 high expression was not an independent prognostic factor for lung adenocarcinoma(P=0.051).Conclusions:Arg-2 is associated with the proliferation,differentiation,lymph node metastasis and TNM stage of lung adenocarcinoma.The high expression of Arg-2 indicates that the prognosis of lung adenocarcinoma is poor,but it is not an independent prognostic factor.

关 键 词：精氨酸酶-2 肺肿瘤 预后 淋巴结转移 细胞增殖 

分 类 号：R734.2[医药卫生—肿瘤]