响应面法优化凤丹内生真菌发酵工艺探究  

Optimizing the fermentation process of dominant endophytic fungus isolated from Paeonia ostii by response surface method

作　　者：张培良 许凤清 王国凯 王刚 马宗慧 刘海涛 韩婧 吴娟 吴培云 ZHANG Peiliang;XU Fengqing;WANG Guokai;WANG Gang;MA Zonghui;LIU Haitao;HAN Jing;WU Juan;WU Peiyun(Phaymacy School,Anhui University of Chinese Medicine,Anhui Engineering Research Center of Modernized Chinese Material,Heifei,Anhui 230012,China)

机构地区：[1]安徽中医药大学药学院现代中药安徽省工程技术研究中心,安徽合肥230012

出　　处：《安徽医药》2018年第12期2319-2322,共4页Anhui Medical and Pharmaceutical Journal

基　　金：安徽省高校自然科学基金重点项目(KJ2017A294)

摘　　要：目的研究响应面法优化一株凤丹优势内生真菌发酵工艺。方法在单因素试验结果的基础上,运用响应面法设计,对凤丹优势内生真菌发酵工艺进行优化。结果最优发酵工艺为蔗糖含量20 g·L^(-1),酵母浸膏含量5 g·L^(-1),KH_2PO_3含量1. 00 g·L^(-1),K_2HPO_3含量1. 00 g·L^(-1)。在该条件下,发酵所得的菌丝体实际生物量为1. 647 g。结论实验所得数据的多元回归模型与实际情况较好拟合,表明该优化工艺稳定可靠,通过响应面法最终获得了凤丹内生真菌最优发酵工艺。Objective Optimizing the fermentation process of one dominant endophytic fungus that separated from Paeonia ostii by response surface method.Methods On the basis of single factor experiment results,optimizing the fermentation process of dominant endophytic fungus separated from Paeonia ostii through using the response surface method design.Results Sucrose content 20 g·L-1,yeast extract content 5 g·L-1,KH 2PO 3 content 1.00 g·L-1,and K 2HPO 3 content 1.00 g·L-1 is the best fermentation process.And the actual biomass of mycelium from fermentation is 1.647 g at that condition.Conclusion The regression model of the data obtained from the experiment can better fitting with actual situation,and the optimized process is stable and reliable,and the best process for the fermentation of strain Fusarium oxysporum from Paeonia ostii was described through the response surface method in this study.

关 键 词：响应面法 凤丹 内生真菌 发酵工艺 

分 类 号：R28[医药卫生—中药学]