表面活性剂对出芽短梗霉发酵生产聚苹果酸的影响  

Effect of Surfactants on Poly Malic Acid Fermentation of Aureobasidium pullulans

作　　者：赵廷彬 陈畅 殷海松[2] 孙爱友[3] 乔长晟[1,4] ZHAO Ting-bin;CHEN Chang;YIN Hai-song;SUN Ai-you;QIAO Chang-sheng(College of Biotechnology,Tianjin University of Science&Technology,Tianjin 300457,China;School of Bioengineering,Tianjin Modern Vocational Technology College,Tianjin 300350,China;State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China;Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control,Tianjin 300457,China)

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]天津现代职业技术学院生物工程学院,天津300350 [3]华东理工大学生物反应工程国家重点实验室,上海200237 [4]天津市微生物代谢与发酵过程控制技术工程中心,天津300457

出　　处：《食品研究与开发》2018年第23期13-18,共6页Food Research and Development

基　　金：天津市科技支撑计划项目(17YFCZZC00310);天津市微生物代谢与发酵过程控制技术工程中心项目(ZXKF20180301)

摘　　要：探究不同表面活性剂对出芽短梗霉CGMCC3337合成聚苹果酸的影响,并对最佳影响因子的作用机制做初步探究。结果发现,十二烷基硫酸钠(sodium dodecyl sulfate,SDS)和曲拉通X-100对聚苹果酸的合成有抑制作用;吐温80和吐温60在低浓度时有利于聚苹果酸的合成,最适添加量均为2 g/L;添加吐温80后,聚苹果酸的产量为31.36 g/L,较对照组提高30.5%。Influence of different surfactants on the synthesis of poly malic acid by Aureobasidium pullulans CGMCC3337 and the mechanism of action of the best influencing factors were studied.The results showed that sodium dodecyl sulfate(SDS)and Triton X-100 had an inhibitory effect on the synthesis of poly malic acid;Tween 80 and Tween 60 were favorable for the synthesis of poly malic acid at low concentrations,and the optimal amount was 2 g/L.After adding Tween 80 to the media,the yield of poly malic acid was 31.36 g/L,with an increase of 30.5%when compared to the control.

关 键 词：出芽短梗霉 聚苹果酸 表面活性剂 脂肪酸 

分 类 号：TQ921[轻工技术与工程—发酵工程] TQ423