阳荷挥发油提取工艺优化及GC-MS分析  

Optimum Extraction Process and GC-MS Analysis of Volatile Oil from Zingiber striolatum Diels

作　　者：蔡依 徐津林 郭百臻 李肖 段金辉 阮金兰[1,2] CAI Yi;XU Jin-lin;GUO Bai-zhen;LI Xiao;DUAN Jin-hui;RUAN Jin-lan(School of Life Science,Wuchang University of Technology,Wuhan 430223,Hubei,China;School of Environmental Ecology and Biological Engineering,Wuhan Institute of Technology,Wuhan 430073,Hubei,China)

机构地区：[1]武昌理工学院生命科学学院,湖北武汉430223 [2]武汉工程大学环境生态与生物工程学院,湖北武汉430073

出　　处：《食品研究与开发》2018年第23期49-53,共5页Food Research and Development

基　　金：生物多肽糖尿病药物湖北省协同创新中心项目(鄂教科涵[2012]56号);湖北省生物多肽糖尿病药物工程技术研究中心项目(鄂科技通[2014]72号)

摘　　要：对阳荷挥发油的提取工艺进行优化并鉴定阳荷挥发油化学成分,为阳荷的开发利用提供试验依据。采用单因素试验、多因素多水平正交试验方法和超声辅助蒸馏提取法提取阳荷挥发油,以阳荷挥发油提取率为评价指标,分别研究超声时间、蒸馏时间和料液比对阳荷挥发油提取率的影响,以气相色谱-质谱(gass chromatography-mass spectormetry,GC-MS)结合NIST4.0质谱库分析挥发油化学成分及种类,用峰面积归一化法计算各成分的相对百分含量。阳荷挥发油的最佳提取工艺是超声60 min、蒸馏时间4 h,料液比1∶3(g/mL),提取率达到0.036 8%;鉴定出17个主要化学成分,占挥发油总量的85.074%,其中烯烃类有8种,含量最高的3个成分是棕榈酸(30.508%),β-水芹烯(12.359%)和芥酸酰胺(7.376%)。To optimize the extraction process and identify the chemical constituents of the volatile oil from Z.striolatum Diels,so as to provide experimental basis for the development and utilization of Z.striolatum Diels.Single factor experiment,multi factor and multi level orthogonal experiment and ultrasonic assisted distillation were used to extract volatile oil from Z.striolatum Diels.Taking the extraction rate of the Z.striolatum Diels volatile oil as the evaluation index,the effects of ultrasonic time,distillation time and solid-liquid ratio on the extraction rate of volatile oil were studied respectively,The chemical constituents and types of volatile oils were analyzed by gass chromatography-mass spectormetry(GC-MS)and NIST4.0 mass spectrometry.The relative percentile content of each component were calculated by using peak area normalization method.The optimal extraction process of the volatile oil were sultrasonic 60 min,distillation time 4 h,material liquid ratio 1∶3(g/mL),extraction rate reached 0.036 8%;and identified 17 major chemical composition,which accounted for 85.074%of the total volatile oil,of which were eight kinds of alkenes,and three of the highest content were palmitic acid(30.508%),beta parsley(12.359%)and erucate(7.376%).

关 键 词：阳荷 挥发油 正交试验 气相色谱-质谱 化学成分 

分 类 号：O657.63[理学—分析化学] TQ651[理学—化学]