3-甲基腺嘌呤对低氧状态下上皮性卵巢癌细胞自噬、迁移和侵袭的影响  

Effect of 3-methyladenine on autophagy, migration and invasion in epithelial ovarian cancer cells under hypoxia

作　　者：唐中园[1,2] 张宁 狄文 李卫平[2,3] TANG Zhong-yuan;ZHANG Ning;DI Wen;LI Wei-ping(Department of Obstetrics and Gynecology,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China;Shanghai Key Laboratory of Gynecologic Oncology,Shanghai 200127,China;Department of Obstetrics and Gynecology,Renji Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200127,China)

机构地区：[1]上海交通大学医学院附属瑞金医院妇产科,上海200025 [2]上海市妇科肿瘤重点实验室,上海200127 [3]上海交通大学医学院附属仁济医院妇产科,上海200127

出　　处：《上海交通大学学报（医学版）》2018年第10期1152-1156,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(81101972);2012年上海市慈善癌症研究中心慈善慢跑基金(SCCRC12008);上海市自然科学基金(16ZR1420000)~~

摘　　要：目的·检测3-甲基腺嘌呤(3-methyladenine,3-MA)对低氧状态下上皮性卵巢癌细胞(epithelial ovarian carcinoma,EOC)自噬、迁移和侵袭的影响。方法·不同浓度的3-MA处理低氧环境下EOC细胞,Western blotting检测自噬相关蛋白LC3的表达,透射电子显微镜观察自噬体的生成,噻唑蓝比色法、黏附试验、Transwell迁移试验和Matrigel侵袭试验分别测定EOC细胞的增殖、黏附、迁移和侵袭能力。结果·3-MA可以抑制低氧环境诱导的LC3-Ⅱ表达增加以及自噬体生成。低氧环境下,5.00 mmol/L的3-MA作用24 h可显著降低EOC细胞存活率(P=0.000);2.50 mmol/L的3-MA作用6 h后,细胞的黏附能力显著降低(P=0.007);1.25 mmol/L和2.50 mmol/L的3-MA均可抑制低氧环境下EOC细胞的迁移和侵袭能力(均P<0.01)。结论·3-MA在抑制低氧状态下EOC细胞自噬的同时,具有抗细胞迁移和侵袭的作用。Objective·To investigate the effect of 3-methyladenine(3-MA)on autophagy,migration and invasion of epithelial ovarian cancer(EOC)cells under hypoxia.Methods·Different concentrations of 3-MA were used to treat EOC cells in hypoxic environment.The expression of autophagy-associated protein LC3 was detected by Western blotting.The autophagosome formation was observed by transmission electron microscopy.The proliferation,adhesion,migration and invasion of EOC cells were determined by thiazole blue colorimetry,adhesion test,Transwell migration test and Matrigel invasion test,respectively.Results·3-MA was able to inhibit the increase of LC3-Ⅱand autophagosome formation induced by hypoxia.In hypoxic environment,the survival rate of EOC cells was significantly decreased by 5.00 mmol/L 3-MA for 24 h(P=0.000).The adhesion ability of EOC cells was significantly decreased by 2.50 mmol/L 3-MA for 6 h(P=0.007).1.25 mmol/L and 2.50 mmol/L 3-MA could inhibit the migration and invasion of EOC cells in hypoxic environment(P<0.01).Conclusion·3-MA can inhibit the autophagy,migration and invasion of EOC cells in hypoxic environment.

关 键 词：3-甲基腺嘌呤 自噬 上皮性卵巢癌细胞 低氧 迁移 侵袭 

分 类 号：R737.31[医药卫生—肿瘤]