白藜芦醇对小鼠结肠癌细胞CT26增殖的影响  

Effect of resveratrol on proliferation of CT26 colonic cancer cells

作　　者：黄诗琪 杨梦[1] 童玲[1] 陆红丽[1] 黄旭[1] 许文燮[1] HUANG Shi-qi;YANG Meng;TONG Ling;LU Hong-li;HUANG Xu;XU Wen-xie(Department of Anatomy and Physiology,Shanghai Jiao Tong University College of Basic Medical Sciences,Shanghai 200025,China)

机构地区：[1]上海交通大学基础医学院解剖与生理学系,上海200025

出　　处：《上海交通大学学报（医学版）》2018年第10期1162-1166,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金面上项目(31571180)~~

摘　　要：目的·观察白藜芦醇(resveratrol,RSV)对小鼠结肠癌细胞CT26增殖以及凋亡的影响,并探讨其中相关的分子机制。方法·梯度浓度20、40、60、80、100μmol/L的RSV孵育细胞后,CCK-8试剂盒检测RSV对CT26细胞增殖的影响;Hoechst 33342活细胞染色液检测RSV对细胞凋亡的影响;Western blotting技术对细胞中凋亡相关的蛋白(Bax、Bcl-2),核转录因子(NF-κB)以及Akt、PTEN的表达量进行检测。结果·RSV能够抑制CT26细胞的增殖并促进其凋亡,抑制作用具有浓度依赖性。RSV能使细胞核内的NF-κB蛋白的表达量下调,细胞质内的NF-κB上调;Bax及PTEN蛋白表达量上调,Bcl-2下调,Akt蛋白的磷酸化下调。结论·RSV能够抑制CT26细胞的增殖,其作用可能与上调Bax和PTEN蛋白,下调Bcl-2、Akt磷酸化及NF-κB活性有关。Objective·To investigate the effect of resveratrol(RSV)on proliferation of mouse colonic cancer cells CT26 and its underlying molecular mechanisms.Methods·CT26 cells were incubated with 20,40,60,80 and 100μmol/L RSV respectively for 24 h.The effect of RSV on cell proliferation was tested with CCK8 kits.The effect of RSV on cell apoptosis was detected by living cell dye Hoechst 33342.The plasma protein and nucleoprotein of the cells were extracted separately by using the nuclear protein and cell plasma protein extraction kits.And the expressions of apoptosis related proteins(Bax and Bcl-2),PTEN,NF-κB and Akt were detected by Western blotting.Results·RSV inhibited the proliferation of CT26 cells.The inhibitory effect was dose-dependent.Treatment with RSV promoted the apoptosis of CT26 cells.The expression of NF-κB protein in the cell nucleus was downregulated compared to the control cells,and the expression of NF-κB in the cytoplasm was up-regulated in the CT26 cells after 24 h incubation with RSV.In the CT26 cells after 24 h incubation of RSV,the expressions of Bax and PTEN were up-regulated,the expression of Bcl-2 was down-regulated,and the phosphorylation of Akt protein was down-regulated.Conclusion·RSV can inhibit the proliferation of colon cancer cells in mice,which may be associated with Bax,Bcl-2,NF-κB,p-Akt and PTEN.

关 键 词：白藜芦醇 结肠癌 细胞增殖 凋亡 小鼠 

分 类 号：R735.3[医药卫生—肿瘤]