β-内酰胺酶在胃癌中的表达及临床意义  

Expression of lactamase β in gastric cancer and its clinical significance

作　　者：赵雪 吴霖[1] 顾勤 ZHAO Xue;WU Lin;GU Qin(Department of Geriatrics,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China;Changshou Community Health Center,Putuo District,Shanghai 200060,China;Dapuqiao Community Health Center,Huangpu District,Shanghai 200023,China)

机构地区：[1]上海交通大学医学院附属瑞金医院老年病科,上海200025 [2]上海市普陀区长寿街道社区卫生服务中心,上海200060 [3]上海市黄浦区打浦桥街道社区卫生服务中心,上海200023

出　　处：《上海交通大学学报（医学版）》2018年第10期1212-1218,共7页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市黄浦区卫生和计划生育委员会科研基金(HKM201733)~~

摘　　要：目的·研究β-内酰胺酶(lactamaseβ,LACTB)在胃癌中的表达及其对胃癌患者预后的影响。方法·用免疫组织化学法分析LACTB在90对配对的胃癌及癌旁组织中的表达差异。用Kaplan-Meier Plotter数据库分析胃癌组织中LACTB mRNA的表达变化与胃癌预后的关系。Cox模型分析影响胃癌患者总生存期的因素并建立预后预测列线图模型。用STRING数据库分析与LACTB相互作用的蛋白,并对LACTB及其相互作用蛋白标以显著关联的GO(gene ontology)功能类标签和KEGG(Kyoto encyclopedia of genes and genomes)通路。结果·LACTB在胃癌组织中的表达显著低于癌旁组织(P=0.000)。LACTB mRNA低表达预示胃癌患者不良预后,预后预测列线图的一致系数为0.641;LACTB及与其相互作用的38种蛋白参与了5条信号通路,GO注释分类结果表明富集于1类细胞学组分、9类分子功能和2类生物学过程。结论·在胃癌中LACTB低表达是一种不良预后因素。Objective·To investigate the expression level of lactamaseβ(LACTB)in gastric cancer and its influence on the prognosis of gastric cancer patients.Methods·The varied expression of LACTB in 90 pairs of gastric cancer tissue and peritumor tissue was analyzed by immunohistochemistry.The prognostic role of LACTB mRNA in gastric cancer was analyzed by the Kaplan-Meier Plotter database.The factors affecting the overall survival of gastric cancer patients were analyzed with Cox survival model.The prognostic nomogram model was established to predict overall survival of gastric cancer patients.The proteins which interacted with LACTB were analyzed by the STRING database,and they were tagged by the notable associated functional cluster of GO(gene ontology)and KEGG(Kyoto encyclopedia of genes and genomes)pathway.Results·The expression of LACTB in gastric cancer tissue was obviously lower than that in peritumor tissue(P=0.000).The low expression of LACTB mRNA may suggest that the prognosis of gastric cancer patient is poor,and the C-index of nomogram for predicting prognosis of gastric cancer patients was 0.641.A set of LACTB and 38 proteins which interacted with LACTB participated in five kinds of signaling pathway.Results for the classification of GO(gene ontology)annotation demonstrated that the proteins were involved in one kind of cellular component,nine kinds of molecular function and two kinds of biological process.Conclusion·The low expression of LACTB may be a poor-prognostic factor in gastric cancer.

关 键 词：Β-内酰胺酶 胃癌 预后分析 列线图 

分 类 号：R735.2[医药卫生—肿瘤]