HPLC法测定黑木耳中腺苷及尿苷含量  

Determination of Adenosine and Uridine in Auricularia auricula by HPLC

作　　者：郑雪 郑春英[1,2] 吴桐 ZHENG Xue;ZHENG Chun-ying;WU Tong(Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education,Heilongjiang University,Harbin,Heilongjiang 150500;;Key Laboratory of Microbiology,College of Heilongjiang Province,School of Life Sciences,Heilongjiang University,Harbin,Heilongjiang 150080;Key Laboratory of Molecular Biology,College of Heilongjiang Province,School of Life Sciences,Heilongjiang University,Harbin,Heilongjiang 150080)

机构地区：[1]黑龙江大学,农业微生物技术教育部工程研究中心,黑龙江哈尔滨150500 [2]黑龙江大学生命科学学院,黑龙江省普通高校微生物重点实验室,黑龙江哈尔滨150080 [3]黑龙江大学生命科学学院,黑龙江省普通高校分子生物学重点实验室,黑龙江哈尔滨150080

出　　处：《安徽农业科学》2018年第32期173-174,181,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]建立简单、快速同时分析黑木耳中腺苷及尿苷含量的HPLC法。[方法]采用Venusil XBP C18色谱柱,流动相为甲醇-水(15:85),流速为1 m L/min,检测波长为260 nm,柱温为室温。[结果]腺苷及尿苷的线性范围分别为0.02～0.10 mg/m L(r=0.999 9)和0.04～0.20 mg/m L(r=0.999 9),平均加样回收率分别为99.58%(RSD=0.78%)、98.99%(RSD=1.21%)。[结论]该方法简单、快速、准确、重现性好、回收率高,可用于黑木耳中腺苷及尿苷含量的测定,其结果可为其质量控制提供参考。[Objective]To develop a HPLC method for simultaneous determination of adenosine and uridine in Auricularia auricular.[Method]The HPLC system consisted of a Venusil XBP C 18 column with the mobile phase of methanol-water(15∶85)at a flow rate of 1.0 mL/min.UV detector was used,and the detection wavelength was 260 nm.The column temperature was room temperature.[Result]The liner range of adenosine and uridine were 0.02-0.10 mg/mL(r=0.999 9)and 0.04-0.20 mg/mL(r=0.999 9),respectively.The average recoveries were 99.58%(RSD=0.78%)and 98.99%(RSD=1.21%),respectively.[Conclusion]The method is accurate,simple,rapid and reproducible for determination of adenosine and uridine in Auricularia auricular.The determination results can be used as a reference for the quality control for Auricularia auricular.

关 键 词：HPLC 黑木耳 腺苷 尿苷 

分 类 号：TS207[轻工技术与工程—食品科学] R931[轻工技术与工程—食品科学与工程]