华卟啉钠介导的光动力疗法对人舌鳞癌细胞株HSC-4的增殖和迁移能力的影响  

Effects of DVDMS-PDT on Proliferation and Migration of Human Tongue Squamous Carcinoma Cell Line HSC-4

作　　者：黄宇思 张磊[1] 刘乐天 马垚 刘鑫 HUANG Yu-si;ZHANG Lei;LIU Le-tian;MA Yao;LIU Xin(Department of Oral Health Sciences,The Affiliated Stomatological Hospital of Harbin Medical University,Harbin 150001,China;Department of Oral and Maxillofacial Surgery,The Affiliated Stomatological Hospital of Harbin Medical University,Harbin 150001,China)

机构地区：[1]哈尔滨医科大学口腔医学院口腔预防保健科,黑龙江哈尔滨150001 [2]哈尔滨医科大学口腔医学院口腔颌面外科,黑龙江哈尔滨150001

出　　处：《口腔医学研究》2018年第11期1208-1211,共4页Journal of Oral Science Research

基　　金：基金项目哈尔滨医科大学附属第一医院科研基金(编号:2014B19)

摘　　要：目的:探讨华卟啉钠(sinoporphyrin sodium,DVDMS)介导的光动力疗法(photodynamic therapy,PDT)对体外培养的口腔舌鳞状细胞癌(oral tongue squamous cell carcinoma,OTSCC,简称鳞癌)细胞株HSC-4的增殖及迁移能力的影响。方法:检测DVDMS细胞毒性,细胞摄取药物情况,将细胞分为对照组以及不同浓度的DVDMS-PDT处理组(DVDMS孵育浓度分别为0.1、0.5、1.0、2.0、3.0、4.0、5.0、6.0、8.0mg/L),激光能量密度为0.75、1.50、3.00、6.00J/cm2,细胞增殖检测试剂盒(cell counting kit-8,CCK-8)法检测DVDMS-PDT对细胞增殖能力的影响,划痕实验和Transwell小室实验观察细胞迁移能力的变化。结果:DVDMS毒性实验显示单纯药物对细胞毒性较小,联合激光照射后,可显著抑制HSC-4细胞增殖(P均<0.05),与对照组比较,划痕愈合率降低,Transwell小室穿膜细胞数减少(P均<0.05)。DVDMS药物浓度2.0 mg/L,体外孵育6h,光能量密度在3.00J/cm2以上可有效杀伤HSC-4。结论:DVDMS-PDT可有效杀伤舌鳞癌细胞,抑制HSC-4的迁移。光敏剂浓度、孵育时间、光能量密度是影响疗效的重要因素。Objective:To study the proliferation and migration of oral tongue squamous cell carcinoma HSC-4 cells induced by DVDMS-PDT.Methods:To detect the cytotoxicity of DVDMS and the uptake of drugs by cells,the cells were divided into control group and DVDMS-PDT treatment group with different concentrations(0.1,0.5,1.0,2.0,3.0,4.0,5.0,6.0,and 8.0 mg/L,respectively).The laser energy density were 0.75,1.50,3.00,6.00J/cm 2.The effects of different drug concentrations and light energy density on cell viability were analyzed by CCK-8.Scratch assay and Transwell experiment were used to observe the changes of cell migration ability.Results:DVDMS toxicity experiments showed that the single drug had less cytotoxicity,and combined with laser irradiation,it could significantly inhibit the proliferation of HSC-4 cells(P<0.05).Compared with the control group,the healing rate of scratch assay was decreased,and the number of transwell penetrated cells was decreased(P<0.05).DVDMS concentration at 2.0 mg/L,6 hours incubation,and light energy density above 3.00J/cm 2 could effectively kill HSC-4 cells.Conclusion:DVDMS-PDT can effectively kill the tongue squamous cell carcinoma cell line and inhibit the migration of HSC-4 cells.Photosensitizer concentration,incubation time,and light energy density are important factors affecting the therapeutic effect.

关 键 词：华卟啉钠 光动力疗法 舌鳞癌 迁移 

分 类 号：R739.86[医药卫生—肿瘤]