两株长喙壳属真菌挥发性香气成分分析  

The Aroma Components Analysis of Two Ceratocystis Strains

作　　者：张志超 张彧[1] 李建梅 徐可成 宋红 李雪 杨虹 黄琼[1] ZHANG Zhi-chao;ZHANG Yu;LI Jian-mei;XU Ke-cheng;SONG Hong;LI Xue;YANG Hong;HUANG Qiong(State Key Laboratory of Conservation and Utilization of Biology Resources of Yunnan Province, Yunnan Agriculture University,Kunming,Yunnan 650201,China;Pesticide Inspection and Identification Institute of Yunnan,Kunming 650034,China)

机构地区：[1]云南农业大学省部共建云南生物资源保护与利用国家重点实验室,昆明650201 [2]云南省农药检定所,昆明650034

出　　处：《天然产物研究与开发》2018年第11期1939-1942,1956,共5页Natural Product Research and Development

基　　金：国家自然科学基金(31860522);福建农林开放项目(NYST-2017-03)

摘　　要：长喙壳属真菌具有产香特性,本文比较了长喙壳菌株PP1-1PG、WYB的菌落形态、r DNA-ITS序列等特征,并利用气相色谱-质谱(GC-MS)联用技术分析其有机化合物组成。结果表明,菌株在PSA培养基上菌丝不发达,为有隔菌丝。菌株PP1-1PG在ITS基因序列系统进化树上与Ceratocystis fimbriata聚为一支,菌株WYB与Ceratocystis paradoxa聚为一组。GC-MS分析表明,两个菌株产生的挥发性香气物质组成主要为乙酸乙酯、乙酸异丁酯、苯乙烯等,其中乙酸乙酯、乙酸异丁酯相对含量最高,二者之和在两个菌株中分别达到45. 85%与61. 02%。本研究首次在菌株PP1-1PG中检测到重要的香料物质苯甲醛(安息香醛),为开发利用苯甲醛提供了参考依据。The genus Ceratocystis produces a range of flavoring compounds,in this study,strains PP1-1PG and WYB were compared on the basis of characteristics of colony morphology and ITS sequences.The volatile components were analyzed by gas chromatography-mass spectrometry(GC-MS).Septate mycelia were observed on PSA substrates for both strains.Based on the ITS phylogeny,strain PP1-1PG was clustered with Ceratocystis fimbriata-forming a distinct clade while strain WYB was clustered with Ceratocystis paradoxa.Analyzing the volatile components by GC-MS,it is known that the most materials produced by two strains were ethyl acetate,isobutyl acetate and styrene,which the relative contents of ethyl acetate and isobutyl acetate were 45.85%and 61.02%in two strains.Benzaldehyde,a widely used flavour substance,was detected from strain PP1-1PG for the first time,providing chemical basis and foundation for the comprehensive development and the search for this substance.

关 键 词：长喙壳属真菌 ITS序列 挥发性物质 GC-MS分析 

分 类 号：R284.1[医药卫生—中药学] Q939.95[医药卫生—中医学]