响应面法优化苦丁茶熊果酸提取工艺及其抑菌活性研究  

Optimization of Extraction of Ursolic Acid from Ilex kudingcha C. J. Tseng by Response Surface Methodology and Its Antibacterial Activity

作　　者：张海全[1] 钟晓坤 黄勤英[1] 许丹妮[1] 农克良[1] ZHANG Hai-quan;ZHONG Xiao-kun;HUANG Qin-ying;XU Dan-ni;NONG Ke-liang(Guangxi Colleges and Universities Key Laboratory Breeding Base of Chemistry of Guangxi Southwest Plant Resources,Guangxi Normal University for Nationalities,Chongzuo 532200,China)

机构地区：[1]广西民族师范学院广西高校桂西南特色植物资源化学重点实验室培育基地,崇左532200

出　　处：《天然产物研究与开发》2018年第11期1978-1985,共8页Natural Product Research and Development

基　　金：广西民族师范学院科研经费资助项目(2016JX001);广西省教育厅2016年广西高校中青年教师基础能力提升项目(KY2016YB517);大学生创新创业项目(201710604044)

摘　　要：为优化苦丁茶熊果酸的提取工艺,并探讨其抑菌活性。在单因素试验基础上,通过响应面分析法,研究了液料比、提取温度、乙醇浓度对苦丁茶熊果酸得率的影响。以金黄色葡萄球菌、枯草芽孢杆菌、生孢梭菌、铜绿假单胞菌、大肠杆菌、白色念珠菌、黑曲霉为供试菌,探究了苦丁茶熊果酸的抑菌活性及最小抑菌浓度(Minimum inhibitory concentration,MIC)。结果表明,最优提取工艺为:液料比为17∶1(mL/g)、乙醇浓度为83%、提取温度83℃;抑菌实验表明,苦丁茶熊果酸对7种菌均有一定的抑制效果,对大肠杆菌、铜绿假单胞菌的MIC为6. 25 mg/mL,对生孢梭菌、金黄色葡萄球菌、枯草芽孢杆菌的MIC为12. 5 mg/mL,对白色念珠菌、黑曲霉的MIC为25 mg/mL。In order to optimize extraction of ursolic acid(UA)from Ilex kudingcha C.J.Tseng and investigate its antibacterial activity.Based on the results of single-factor tests,the ratio of liquid to solid,extraction temperature and ethanol concentration were optimized by response surface analysis.Staphylococcus aureus,Bacillus subtilis,Clostridium sporogenes,Pseudomonas aeruginosa,Escherichia coli,Candida albicans,Aspergillus niger were used as tested bacterial strains in this experiment to explore antibacterial activity of UA from Ilex kudingcha C.J.Tseng and its MIC.The results demonstrated that the process of optimum extraction were the ratio of liquid to solid of 17∶1(mL/g),ethanol concentration of 83%,extraction temperature of 83℃.The antibacterial experiments showed that UA had a certain inhibition on 7 strains.The MIC was as following:6.25 mg/mL to Escherichia coli and Pseudomonas aeruginosa,12.5 mg/mL to Staphylococcus aureus,Bacillus subtilis,Clostridium sporogenes,25 mg/mL to Candida albicans and Aspergillus niger.

关 键 词：苦丁茶 响应面法 熊果酸 抑菌活性 

分 类 号：R284.2[医药卫生—中药学]