长链非编码RNA MALAT1靶向miR-570-3p对人视网膜母细胞瘤细胞系SO-Rb50增殖、凋亡和侵袭的调控作用  

Long noncoding RNA MALAT1 regulates cell proliferation,apoptosis and invasion of human retinoblastoma cell SO-Rb50 via targeting miR-570-3p

作　　者：闫义涛[1] 王晓丽[1] 谷圆圆 任艳凡 胡俊喜[1] YAN Yi-Tao;WANG Xiao-Li;GU Yuan-Yuan;REN Yan-Fan;HU Jun-Xi(Department of Ophthalmology,First Affiliated Hospital of Xinxiang Medical College,Xinxiang 453003,China)

机构地区：[1]新乡医学院第一附属医院眼科,新乡453003

出　　处：《中国免疫学杂志》2018年第11期1621-1625,1631,共6页Chinese Journal of Immunology

基　　金：河南省科技厅项目(No.112102310212)

摘　　要：目的:本文旨在探究长链非编码RNA(lnc RNA)肺腺癌转移相关转录子1(MALAT1)对人视网膜母细胞瘤细胞系SO-Rb50增殖、凋亡和侵袭的影响及其机制。方法:通过sh RNA沉默lnc RNA MALAT1。SO-Rb50细胞分为4组:sh-Ctrl(对照)组,sh-MALAT1组,mi R-570 inhibitor组和sh-MALAT1+mi R-570 inhibitor组。QRT-PCR检测lnc RNA MALAT1和micro RNA(mi R)-570-3p表达。荧光素实验验证靶向关系。肿瘤成球实验分析细胞增殖能力。流式细胞术检测细胞凋亡。Transwell检测细胞侵袭。结果:lnc RNA MALAT1负向调控mi R-570-3p表达(P<0. 05)。荧光素实验表明Lnc RNA MALAT1可直接靶向mi R-570-3p。sh-MALAT1组细胞数目明显少于对照组。mi R-570 inhibitor组细胞数目明显多于对照组。但与sh-MALAT1组相比,sh-MALAT1+mi R-570 inhibitor组细胞数目明显升高。sh-MALAT1组细胞凋亡率明显高于对照组。MiR-570 inhibitor组细胞凋亡率明显低于对照组。Sh-MALAT1+mi R-570 inhibitor组细胞凋亡率则明显低于sh-MALAT1组。与对照组相比,shMALAT1组平均每个视野下侵袭细胞数目明显减少,mi R-570 inhibitor组平均每个视野下侵袭细胞数目明显变多。ShMALAT1+mi R-570 inhibitor组平均每个视野下侵袭细胞数明显多于sh-MALAT1组。结论:Sh-MALAT1通过mi R-570-3p提高人视网膜母细胞瘤细胞系SO-Rb50凋亡,降低细胞增殖和侵袭。Objective:This study aims to explore the effect of long noncoding RNA(lncRNA)metastasis associated lung adenocarcinoma transcript 1(MALAT1)on the proliferation,apoptosis and invasion of human retinoblastoma cell SO-Rb50.Methods:LncRNA MALAT1 was silence by shRNA.SO-Rb50 cells were divided into four groups:sh-Ctrl(control)group,sh-MALAT1group,miR-570 inhibitor group and sh-MALAT1+miR-570 inhibitor group.The expression of lncRNA MALAT1 and microRNA(miR)-570-3p was detected by qRT-PCR.The targeting relationship of lncRNA MALAT1 and miR-570-3p was verified by luciferase assay.Cell proliferation was measured by tumor sphere formation assay.Apoptosis was tested by flow cytometry.Cell invasion was detected by Transwell.Results:LncRNA MALAT1 negatively regulated the expression of miR-570.Luciferase assay indicated that miR-570 was a direct target of lncRNA MALAT1.Cell numbers of sh-MALAT1 group was less than control group.And the cell numbers of miR-570 inhibitor group was more than control group.But compared with sh-MALAT1 group,the cell numbers of sh-MALAT1+miR-570 inhibitor group was increased.Cell apoptosis rate in sh-MALAT1 group was higher than control group.Cell apoptosis rate in miR-570 inhibitor group was lower than control group.Cell apoptosis rate in sh-MALAT1+miR-570 inhibitor group was lower than sh-MALAT1 group.Conclusion:Compared with control group,the mean invasive cells per field in sh-MALAT1 group were reduced and the mean invasive cells per field in miR-570 inhibitor group were enhanced.The mean invasive cells per field in sh-MALAT1+miR-570 inhibitor group were more than sh-MALAT1 group.Sh-MALAT1 elevates the apoptosis and reduces cell proliferation and invasion of human retinoblastoma cell SO-Rb50 via miR-570-3p.

关 键 词：lncRNA MALAT1 miR-570-3p 视网膜母细胞瘤 增殖 凋亡 侵袭 

分 类 号：R739.72[医药卫生—肿瘤]