水稻细菌性穗枯病菌分离条件的优化及产毒黄素观察  

Optimization of Burkholderia glumae isolation and observation oftoxoflavin production

作　　者：徐以华 孙磊[1,2] 王玲 刘连盟 梁梦琦 高健 黎起秦 侯雨萱[2] 黄世文 XU Yihua;SUN Lei;WANG Ling;LIU Lianmeng;LIANG Mengqi;GAO Jian;LI Qiqin;HOU Yuxuan;HUANG Shiwen(College of Agronomy,Guangxi University,Nanning 530003,China;State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 310006,China)

机构地区：[1]广西大学农学院,南宁530003 [2]中国水稻研究所,水稻生物学国家重点实验室,杭州310006

出　　处：《植物保护》2018年第6期38-44,共7页Plant Protection

基　　金：中国农业科学院科技创新工程(CAAS-ASTIP-2013-CNRRI);国家重点研发计划(2016YFD0200801)。

摘　　要：水稻穗枯病是水稻生产上重要的细菌性病害,目前针对其病原菌快速高效分离的体系很少有报道。本文以水稻细菌性穗枯病菌Burkholderia glumae标准菌株LMG2196为研究对象,探讨分析了B.glumae的生长温度、培养基上的菌落形态及对抗生素敏感性等。结果表明,选用含50μg/mL氨苄青霉素的KB平板划线,25℃培养36 h,可以快速高效分离B.glumae。B.glumae主要通过分泌毒黄素对寄主产生致病性,本文利用分光光度法测定了LMG2196菌株在不同温度以及营养条件下毒黄素的产生。LMG2196菌株在25～40℃之间均能产生毒黄素,在营养条件不同的LB和PPG液体培养基中,同一温度下,总体上表现为在PPG中产生的毒黄素要比LB中的多;且LB的最适产毒黄素温度是28～30℃,PPG的是37℃,表明毒黄素的产生与温度和营养条件有关。Bacterial panicle blight is one of the destructive diseases on rice crop.However,there are few studies on rapid and efficient isolation of the pathogen Burkholderia glumae.Growth temperature,colony morphology and antibiotic resistance of standard strain of B.glumae LMG2196 were investigated in this study.The results showed that LMG2196 was rapidly and effectively isolated on KB medium with the concentration of 50μg/mL ampicillin cultured 36 h at 25℃.Furthermore,the effects of temperature and nutrition on the production of toxoflavin from LMG2196 were also studied by spectrometry detection.The results confirmed that LMG2196 could produce toxoflavin at the temperature from 25℃to 40℃in both LB and PPG liquid medium.The level of toxoflavin production in PPG was higher than in LB at the same temperature.The optimal toxoflavin-producing temperatures were 28℃to 30℃in LB medium and 37℃in PPG medium.All the above results showed that the production of the toxoflavin was correlated to temperature and nutrition.

关 键 词：水稻细菌性穗枯病 病原菌 快速高效分离 毒黄素 

分 类 号：S435.111.49[农业科学—农业昆虫与害虫防治]