miR-26a靶向HMGA1基因对结肠癌细胞生长、侵袭和迁移的影响  

Effects of miR-26 atargeting HMGA1 gene on growth,invasion and migration of colon cancer cells

作　　者：王廷刚[1] 薛峰[2] 李宇[2] 牛兆健[2] 王野[1] WANG Tinggang;XUE Feng;LI Yu;NIU Zhaojian;WANG Ye(Department of General Surgery,Qingdao Haici Medical Group,Qingdao 266000,China;Department of General Surgery,Affiliated Hospital,College of Medical Sciences,Qiingdao University,Qingdao 266000,China)

机构地区：[1]青岛海慈医疗集团普外科,山东青岛266000 [2]青岛大学医学院附属医院普外科,山东青岛266000

出　　处：《吉林大学学报（医学版）》2018年第6期1205-1211,共7页Journal of Jilin University：Medicine Edition

基　　金：山东省科技厅自然科学基金资助课题(ZR2014HL108)

摘　　要：目的:探讨miR-26a靶向高迁移率族蛋白1 (HMGA1)基因对结肠癌细胞生长、侵袭和迁移的影响,阐明HMGA1基因是否为miR-26a的靶基因。方法:将miR-NC (对照)、miR-26amimics (模拟物)和miR-26ainhibitor (抑制物)转染人结肠癌SW480细胞作为miR-NC组、miR-26amimics组和miR-26ainhibitor组。RT-PCR和Western blotting法分别检测各组SW480细胞中HMGA1mRNA和蛋白表达水平。采用荧光素酶报告基因检测试剂盒检测各组SW480细胞中双荧光素酶活性,以此确定HMGA1是否为miR-26a的靶基因。采用CCK8实验检测各组细胞增殖活力,Transwell小室法检测各组细胞侵袭数和迁移数。结果:HMGA1基因是miR-26a的靶基因。与miR-NC组比较,转染24、48和72h时miR-26amimics组SW480细胞增殖活力明显降低(P<0.01),而miR-26ainhibitor组细胞增殖活力明显升高(P<0.01)。与miR-NC组比较,各时间点miR-26amimics组和miR-26amimics+pcDNA3.1-HMGA1组细胞增殖活力、细胞侵袭数和细胞迁移数均降低(P<0.01),而miR-NC+pcDNA3.1-HMGA1组细胞增殖活力、细胞侵袭数和细胞迁移数均升高(P<0.01);与miR-26amimics组比较,各时间点miR-26amimics+pcDNA3.1-HMGA1组细胞增殖活力、细胞侵袭数和细胞迁移数均升高(P<0.01);与miR-NC+pcDNA3.1-HMGA1组比较,各时间点miR-26a+pcDNA3.1-HMGA1组细胞增殖活力、细胞侵袭数和细胞迁移数均降低(P<0.01)。结论:HMGA1是miR-26a的靶基因。上调miR-26a表达可抑制人结肠癌SW480细胞增殖,下调miR-26a表达可促进人结肠癌SW480细胞增殖。Objective:To investigate the effects of miR-26a targeting high mobility group protein 1(HMGA1)gene on the growth,invasion and migration of colon cancer cells,and to clarify whether the HMGA1 gene was the target gene of miR-26a.Methods:The miR-NC(miR-NC group),miR-26a mimics(miR-26a mimics group)and miR-26a inhibitor(miR-26a inhibitor group)were transfected into the human colon cancer SW480 cells.RT-PCR and Western blotting methods were used to detect the mRNA and protein expression levels of HMGA1.Luciferase reporter gene detection kit was used to detect the double luciferase activity in SW480 cells and to determine whether HMGA1 was the target gene of miR-26a.The cell proliferation activities of cells in various groups were detected by CCK8 assay;the number of invasion and migration cells was detected by Transwell chamber method.Results:HMGA1 gene was the target gene of miR-26a.Compared with miR-NC group,the proliferation activities of colon cancer SW480 cells in miR-26a mimics group at after 24,48 and 72 h after transfection were significantly decreased(P<0.01),while the proliferation activities of colon cancer SW480 cells in miR-26a inhibitor group were significantly increased(P<0.01).Compared with miR-NC group,the proliferation activities of colon cancer SW480 cells,the number of invasion and migration cells in miR-26a mimics group and miR-26a mimics+pcDNA3.1-HMGA1 group at different time points were significantly decreased(P<0.01);the proliferation activities of colon cancer SW480 cells,the number of invasion and migration cells in miR-NC+pcDNA3.1-HMGA1 group were increased(P<0.01).Compared with miR-26a mimics group,the proliferation activities of colon cancer SW480 cells,the number of invasion and migration cells in miR-26a mimics+pcDNA3.1-HMGA1 group at different time points were increased(P<0.01);compared with miR-NC+pcDNA3.1-HMGA1 grou p,the proliferation activities of colon cancer SW480 cells,the number of invasion and migration cells in miR-26 mimics+pcDNA3.1-HMGA1 group at different time points

关 键 词：miR-26a 高迁移率族蛋白1 结肠肿瘤 增殖 侵袭 迁移 

分 类 号：R735.35[医药卫生—肿瘤] Q253[医药卫生—临床医学]