一株好氧反硝化菌的鉴定及脱氮特性研究  

Identification of an Aerobic Denitrifier and Its Denitrification Characteristics

作　　者：李雪[1] 刘思彤[2] 陈倩[2] LI Xue;LIU Sitong;CHEN Qian(School of Chemistry and Environmental Engineering,Lanzhou City University,Lanzhou 730070;Beijing Engineering Research Center of Advanced Wastewater Treatment,Department of Environmental Engineering,Peking University,Beijing 100871)

机构地区：[1]兰州城市学院化学与环境工程学院,兰州730070 [2]北京大学环境工程系,北京市新型污水深度处理工程技术研究中心,北京100871

出　　处：《北京大学学报（自然科学版）》2018年第6期1276-1282,共7页Acta Scientiarum Naturalium Universitatis Pekinensis

基　　金：北京市科技专项(Z161100005016034)资助。

摘　　要：以筛选分离得到的好氧反硝化菌HG-7为研究对象,经过16SrRNA同源性分析,初步鉴定该菌株为假单胞菌属(Pseudomonassp.)。对菌株HG-7反硝化功能基因的扩增结果表明,菌体HG-7内存在好氧反硝化功能基因napA和nirK,证实该细菌为好氧反硝化细菌。对菌株的脱氮特性和影响因素的研究表明,以硝酸盐氮为氮源时,菌株的最适碳源为乙酸钠和丁二酸钠,最佳C/N比为6～10,最适宜的温度范围为26～30℃。在上述条件下,菌株HG-7的好氧反硝化活性较高,48小时内对100 mg/L硝酸盐氮的去除率可达98%,且在反应过程中亚硝酸盐氮积累量较低。以亚硝酸氮为唯一氮源时,低浓度条件下可实现100%的氮素去除率;高浓度条件下,脱氮速率则受到明显的抑制,对91.4 mg/L的亚硝酸盐氮氮去除率约为40%。因此,将该菌株应用于废水的脱氮处理,可实现氮素的有效去除,具有潜在的应用价值。Strain HG-7 was identified as Pseudomonas sp.through 16s rRNA gene sequence analysis.The aerobic denitrification ability of strain HG-7 was further proved by the existence of the napA and nirK genes.Further studies showed that the optimal carbon resources for strain HG-7 were sodium acetate and sodium succinate,the optimal C/N ratio was 6?10,and the suitable temperature was 26?30℃.At this condition,with an initial nitrate nitrogen of 100 mg/L,98%of nitrate could be removed in 48 hours and the amount of nitrite accumulation was very small.Moreover,with nitrite as the sole nitrogen source,100%of nitrite could be removed at lower nitrite concentration.But the nitrite nitrogen removal rate was about 40%when the nitrite concentration increased to 91.4 mg/L,indicating that high nitrite concentration was harmful for aerobic denitrification by strain HG-7.The strain HG-7 has great potential to be used for biological nitrogen removal,which was particularly significant for actual wastewater treatment processes.

关 键 词：好氧反硝化菌 鉴定 脱氮特性 

分 类 号：X172[环境科学与工程—环境科学]