醒脑再造胶囊对甲醛致小鼠记忆损害的影响  

Ameliorating effect of Xingnaozaizao capsule on formaldehyde-induced memory impairment in mice

作　　者：盛志豪 陈颖 马瑞 陈秋冲 李雅静 王丹 SHENG Zhihao;CHEN Ying;MA Rui;CHEN Qiuchong;LI Yajing;WANG Dan(School of Anesthesiology,Xuzhou Medical University,Xuzhou 221004,China;Department of Anesthetic Pharmacology,Xuzhou Medical University,Xuzhou 221004)

机构地区：[1]徐州医科大学麻醉学院,江苏徐州221004 [2]徐州医科大学麻醉药理学教研室,江苏徐州221004

出　　处：《中国比较医学杂志》2018年第11期42-47,共6页Chinese Journal of Comparative Medicine

基　　金：国家级大学生创新创业训练计划项目(201710313025);江苏省高等学校大学生创新创业训练计划重点项目(201710313025Z);江苏高校品牌专业建设工程资助项目(PPZY2015A066)

摘　　要：目的探讨醒脑再造胶囊对甲醛所致小鼠记忆损害的影响及其作用机制。方法将SPF级KM小鼠随机分为空白对照组,溶剂对照组和低、中、高剂量组5组,每组14只,将除空白对照组以外的4组小鼠分别置于甲醛染毒装置中进行染毒,持续14 d。通过避暗实验检测各组小鼠的学习记忆能力;采用生化方法测定脑组织中一氧化氮合酶(NOS)、超氧化物歧化酶(SOD)活力以及丙二醛(MDA)水平;采用苏木素-伊红染色法观察小鼠海马CAl区锥体细胞的数目及形态结构变化。结果与空白对照组比较,溶剂对照组小鼠避暗潜伏期缩短(P<0.01),错误次数增加(P<0.01),脑组织中NOS和SOD活力降低(P<0.05),MDA水平升高(P<0.01),海马CA1区锥体细胞数目减少,排列稀疏紊乱,形态不规则。与溶剂对照组比较,中、高剂量组小鼠潜伏期均延长(P<0.05),NOS和SOD活力均升高(P<0.01),低、中、高剂量组小鼠错误次数均减少(P<0.05),MDA水平均降低(P<0.01)。低、中剂量组小鼠海马CA1区锥体细胞层数略有增多,高剂量组海马CA1区锥体细胞层次清楚,排列紧密,数量明显增多。结论醒脑再造胶囊可改善甲醛所致的小鼠记忆损害,其机制可能与其抗氧化损伤作用及增加海马CA1区锥体细胞的数目有关。Objective To observe the effects of Xingnaozaizao capsule on formaldehyde-induced impairment of memory and preliminarily explore the mechanisms in mice.Methods Specific pathogen-free healthy KM mice were randomly divided into blank control,solvent control,and low-,medium-and high-dose groups with 14 mice in each group.The groups of mice,except the blank control group,were placed in a formaldehyde poisoning device for 14 days.The latency and number of errors in a step-through test were observed.The activities of nitric oxide synthase(NOS)and superoxide dismutase(SOD),and the content of malonaldehyde(MDA)in brain tissue were measured biochemically.The number and morphology of pyramidal neurons in the mouse hippocampal CA1 area were observed after HE staining.Results Compared with the blank control group,the latency was shortened(P<0.01),the number of errors was increased(P<0.01),NOS and SOD activities in brain tissue were decreased(P<0.05),and the content of MDA was increased(P<0.01)in the solvent control group.The numbers of piramidal cells were reduced,cell arrangement was sparse and disordered,and the cell shape was irregular in the CA1 area of the hippocampus in the solvent control group.Compared with the solvent control group,the latencies of medium-and high-dose groups were lengthened(P<0.05)and the numbers of errors in low-,medium-and high-dose groups were decreased(P<0.05).The activities of NOS and SOD were increased in brain tissues of medium-and high-dose groups(P<0.01).The contents of MDA were decreased in brain tissues of low-,medium-and high-dose groups(P<0.01).The layers of pyramidal neurons in the mouse hippocampal CA1 area were increased slightly in the low-and medium-dose groups.The layers of pyramidal neurons were clear,cell arrangement was compact,and the numbers of cells were increased in the CA1 area of the hippocampus in the high-dose group.Conclusions Xingnaozaizao capsule improves formaldehyde-induced impairment of memory in mice.Its mechanisms may be associated with the effects of antioxida

关 键 词：醒脑再造胶囊 甲醛 一氧化氮合酶 超氧化物歧化酶 丙二醛 海马 小鼠 

分 类 号：R-33[医药卫生]