裸鼠原位肝癌动态影像检查与病理对照研究  

Scheduled imaging and pathological analysis of an orthotopic hepatic carcinoma model in nude mice

作　　者：沈智勇[1] 陈瑜凤[1] 邵晶晶[1] 沈爱军[1] 丁勇生[1] 李君[1] SHEN Zhiyong;CHEN Yufeng;SHAO Jingjing;SHEN Aijun;DING Yongsheng;LI Jun(Department of Imaging,Nantong Tumor Hospital,Nantong 226361,China)

机构地区：[1]南通市肿瘤医院影像科,江苏南通226361

出　　处：《中国比较医学杂志》2018年第11期74-80,共7页Chinese Journal of Comparative Medicine

基　　金：江苏省六大人才高峰项目(2015-WSW-081);南通市科技计划项目(MS12016033)

摘　　要：目的影像学动态监测裸鼠原位肝癌建模后肿瘤生长并与病理对照,判断干预治疗的合适时期。方法 HepG2细胞注射裸鼠皮下,成瘤后切成1 mm^3组织块,植入15只裸鼠肝内。建模后第4、6、8周分别对5只裸鼠进行超声、磁共振成像(magnetic resonance imaging,MRI)检查,测量肿瘤长径,完毕麻醉处死裸鼠,解剖观察肝成瘤状况,测量肿瘤长径,标本送病理。结果裸鼠肝癌成瘤率80%,肿瘤均为单个,边界清晰。超声检出率75%,MRI检出率100%。4周时肿瘤平均长径4 mm,6周时平均长径7 mm,8周时平均长径11 mm。Wilcoxon秩和检验,超声与解剖比较,P=0.0059,MRI与解剖比较,P=0.208。4、6周时监测,肝癌超声表现均匀低回声;T2WI表现为边界清晰、均匀一致高信号;病理表现瘤细胞致密、核大、异型性明显。8周时监测,超声表现为不均匀低回声;T2WI为形态不规则高信号,中心杂乱、分布不均;病理表现为肿瘤内部坏死。结论组织块建模裸鼠原位肝癌成瘤率高。影像学特征与病理表现相关。MRI是全程监测肿瘤生长的有效影像学方法。1 mm^3瘤块接种裸鼠肝,4～6周可能是进行干预治疗的最佳时期。Objective Periodical imaging to monitor tumor growth in nude mice by pathology after establishment of an orthotopic liver cancer model to determine the appropriate treatment time.Methods HepG2 hepatoma cell line was injected into nude mice subcutaneously.The subcutaneous tumors were then cut into 1 mm3 pieces and implanted into the livers of 15 nude mice.After model establishment,five nude mice in each group were examined by ultrasound(US)and magnetic resonance imaging(MRI),and the longitudinal diameter of the tumors was calculated at weeks 4,6 and 8.Then,the nude mice were sacrificed and anatomized by an anesthetic overdose for observation of liver tumor formation,longitudinal diameter calculation,and pathological examination.Results An 80%tumor formation rate was observed in the nude mouse liver with uniform,focal,and solitary tumors.The detection rates of US and MRI were 75%and 100%respectively.The average longitudinal diameter of the tumors at 4,6,and 8 weeks were 4,7,and 11 mm,respectively.The Wilcoxon rank sum test revealed a significant difference between US and anatomical calculation(P=0.0059),and no significant difference between MRI and anatomical calculation(P=0.208).At 4 and 6 weeks,the liver tumors in nude mice manifested with a homogeneous hypoecho in US,regular morphology and uniform strong signals with a clear boundary in T2WI,and compact atypical tumor cells with a large nucleus in pathological observation.At 8 weeks,the tumors presented with a heterogeneous echo in US,irregular shape and heterogeneous strong signals in T2WI,and obvious central necrosis in pathological observation.Conclusions Intrahepatic tissue implantation is effective for mouse model establishment of orthotopic hepatic carcinoma.The imaging features are correlated with the pathological findings.MRI is an effective imaging method to monitor the tumor growth throughout the entire course.Between 4 and 6 weeks after 1 mm3 tumor tissue inoculation into the nude mouse liver may be the optimum period for initial tumor treatment.

关 键 词：裸鼠 肝 原位癌 模型 超声 磁共振成像 

分 类 号：R-33[医药卫生]