SD大鼠肢体缺血-再灌注损伤模型的建立  

Establishment of a Sprague-Dawley rat model of limb ischemia-reperfusion injury

作　　者：申震 朱付平[1] 潘成熙 罗伟业 周富强 李武平[1] SHEN Zhen;ZHU Fuping;PAN Chengxi;LUO Weiye;ZHOU Fuqiang;LI Wuping(The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,China)

机构地区：[1]湖南中医药大学第一附属医院,长沙410007

出　　处：《中国比较医学杂志》2018年第11期100-105,共6页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金(81674008)

摘　　要：目的介绍一种改良的体外闭合阻断股动脉血流的方法,建立简便可控、质量可靠的肢体缺血-再灌注损伤(limb ischemia-reperfusion injury,LIRI)大鼠模型。方法 15只2月龄SPF级SD雄性大鼠随机分为5个组:正常组,缺血再灌注0 h组、2 h组、4 h组和8 h组。正常组不阻断血流,其余4组构建缺血-再灌注损伤模型,采用HE染色法观察骨骼肌细胞形态学变化,DAPI法检测细胞凋亡。结果除正常组外,各组均出现不同程度肌纤维肿胀、排列不规则、肌纤维间隙增宽等情况,4 h和8 h两组损伤程度较之0 h和2 h两组显著加重,8 h组损伤程度最重,且四组骨骼肌细胞表现出典型的凋亡形态学特征。结论此方法能反映LIRI过程中骨骼肌的病理变化特点,与LIRI临床特征相似,操作简便、高效,可用于肢体缺血-再灌注动物模型的构建。Objective To describe an improved method of blocking blood flow in the femoral artery on the body surface and establish a simple and controllable rat model of limb ischemia-reperfusion injury with reliable quality.Methods A total of 15 SD rats were randomly divided into five groups including the normal control group,and 0,2,4,and 8 h reperfusion groups after ischemia.After establishing the limb ischemia-reperfusion injury model,skeletal muscle morphology and apoptosis of skeletal muscle cells were observed by HE and DAPI staining,respectively.Results Except in the normal group,each group showed various degrees of muscle fiber swelling and irregular arrangements as well as widened inter-myofiber space.Compared with 0 and 2 h groups,muscle fiber swelling,rupture,inflammatory cell infiltration,and other damages in the 4 and 8 h groups were more serious,and the 8 h group had the most serious symptoms.Moreover,skeletal muscle cells of the four groups showed typical apoptotic morphological characteristics.Conclusions This method can reflect the pathological changes of skeletal muscle during LIRI,which are similar to the clinical features of LIRI.It is simple to perform and can be used to establish a limb ischemia-reperfusion animal model.

关 键 词：肢体缺血-再灌注损伤 动物模型 SD大鼠 骨骼肌 

分 类 号：R-33[医药卫生]