云南水稻品种与美国光壳稻构建的重组近交系的籽粒与糙米相关性状遗传分析  

Genetic Analysis of Grain and Brown Rice Related Traits Based on the Recombinant Inbred lines Constructed by Yunnan Local Rice and American Nuda Rice

作　　者：白和灵 李明波 孔芹 尤丽 姚贵燕 谭亚玲[1,2,3] 徐津 BAI Heling;LI Mingbo;KONG Qin;YOU Li;YAO Guiyan;TAN Yaling;XU Jin(Rice Research Institute,Yunnan Agricultural University,Kunming 650201,China;College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China;Yunnan Engineering Research Center for Japonica Hybrid Rice,Kunming 650201,China)

机构地区：[1]云南农业大学稻作研究所,云南昆明650201 [2]云南农业大学农学与生物技术学院,云南昆明650201 [3]云南省杂交粳稻工程技术研究中心,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2018年第5期783-789,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省重点科技项目(2014BB016)

摘　　要：【目的】籽粒和糙米及其相关性状影响稻米品质与产量,而这些性状多属于数量性状。本研究利用合适的分离群体研究它们的遗传规律,以便后期进一步定位、克隆相关基因。【方法】以来源于美国的光壳稻US19和云南高海拔粳稻TJ15为亲本杂交,采用单粒传法构建1个具有90个株系的重组近交系(RILs)群体,考察该群体的籽粒和糙米相关性状,利用相关分析和通径分析等方法研究其遗传规律。【结果】籽粒和糙米长宽及其长宽比和粒重等性状在重组近交系的株系间存在广泛的变异,且有大量的超亲株系产生;各性状均呈连续分布,符合数量性状遗传规律;籽粒的千粒重与籽粒的粒长、粒宽显著相关(P<0.05),籽粒的千粒重和糙米的粒长、粒宽也显著相关(P<0.05)。通径分析表明:影响千粒重最主要的因素是籽粒宽和长宽比;糙米的粒型决定于籽粒的形状,而千粒重是影响糙米率的重要因素。【结论】以美国的光壳稻US19和云南高海拔粳稻TJ15构建的重组近交系群体内存在广泛的遗传重组变异,除粒宽和长宽比性状之外还有其他性状对籽粒千粒重有较大的作用,细长粒型和短圆粒型的水稻均可能有较高的出糙率,为下一步开展籽粒灌浆特性与出糙率关系的研究和创制优异种质材料奠定了基础。[Purposes]Rice grain and brown rice related traits are the important factors that influence the yield and quality of rice.And those traits are all quantitative traits.Study on the genetic pattern of those traits based on appropriate genetic segregation population,is the basis of mapping and cloning related function genes.[Methods]A recombinant inbred line(RIL)containing 90 lines were constructed with American Nuda Rice,US19 and a Yunnan Local Rice variety,TJ15 as parents by single seed descent method.And the genetic pattern of grain and brown rice related traits were researched using correlation and path analysis based on the data of RIL.[Results]The grain and brown rice traits,such as grain length(GL),grain width(GW),length-width ratio(L/W),brown rice length(BRL),brown rice width(BRW),brown rice length-width ratio(BL/W),thousand grain weight(TGW)and brown rice ratio(BRR)were continuous distribution,accord with quantitative trait feature.All studying traits were wide-ranging variation,and exited a lot of super-parent lines in RIL.Correlation analysis showed that TGW was positive related GL,GW,BGL and BGW(P<0.05).Path analysis showed that GW and L/W were the main traits which influence the TGW.The shape of brown rice is depended by the grain shape,and the brown rice ratio is mainly related with TGW.[Conclusion]There is a wide range of genetic recombination variation in the recombinant inbred line population derived from American Nuda Rice,US19 and a Yunnan Local Rice variety,TJ15.Except GW and grain length-width ratio,there are other traits that have a significant effect on grain weight,high BRR can be found in both slender grain type rice and short round grain rice,and this is the basis for study the relationship between BRR and grain filling feature based our data and create excellent germplasm materials.

关 键 词：水稻 重组近交系 籽粒 糙米 通径分析 

分 类 号：S511.033[农业科学—作物学]