血清外泌体miRNA-193b诊断阿尔茨海默病的价值  

Value of serum exosomal miR-193b detection in diagnosis of Alzheimer′s disease

作　　者：刘辰庚[1] 杨婷婷[1] 王培昌[1] LIU Chengeng;YANG Tingting;WANG Peichang(Department of Clinical Laboratory,Xuanwu Hospital,Capital Medical University,Beijing 100053,China)

机构地区：[1]首都医科大学宣武医院检验科,北京100053

出　　处：《实用医学杂志》2018年第21期3621-3624,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金青年基金项目(编号:81401734)

摘　　要：目的探讨血清外泌体miR-193b在阿尔茨海默病(AD)早期诊断的应用价值。方法以2015年9月至2016年8月就诊于首都医科大学宣武医院的患者为研究对象,使用荧光定量PCR法检测主观认知障碍(SCD)、轻度认知障碍(MCI)、痴呆期(DAT)患者及正常对照组血清外泌体miR-193b水平并进行组间比较,用ROC曲线来评价分析miR-193b对SCD的诊断能力。结果 SCD、MCI和DAT患者血清外泌体内miR-193b水平均低于对照组,且DAT组低于MCI组,MCI组低于SCD组,差异有统计学意义(P <0.05)。诊断SCD时,AUC为0.947(95%CI:0.886～0.981),当诊断临界值为1.83时,特异度为94.7%,灵敏度为83.3%。结论血清外泌体miR-193b有望成为AD早期诊断的生物标志物。Objective To investigate the clinical value of serum exosomal miR-193b in the early diagnosis of Alzheimer′s disease.Methods The patients were collected in Xuanwu Hospital of Capital Medical University between September 2015 and August 2016.The levels of miR-193b were detected with quantitative real time polymerasechain reaction(qRT-PCR).Results The exosomal miR-193b expression in the serum in patients with the subjective cognitive decline(SCD),mild cognitive impairment(MCI)and patients with dementia of Alzheimer′s type(DAT)was significantly reduced(P<0.05).Its expression in MCI group was higher than that in DAT group(P<0.05)and lower than that in SCD group(P<0.05).The optimal cut-off value of miR-193b was 1.83,and its sensitivity and specificity were 94.7%and 83.3%respectively in the diagnosis of SCD.Conclusion The serum exosomal miR-193b is likely to become a biomarker in early diagnosis of AD.

关 键 词：外泌体 血清 阿尔茨海默病 诊断标志物 miR-193b 

分 类 号：R749.16[医药卫生—神经病学与精神病学]