基于生物信息学方法分析甲酰肽受体1在胶质瘤中的表达及临床意义  

Bioinformatic analysis of expression and clinical significance of formyl peptide receptor 1 in gliomas

作　　者：董强 解琪琪 胡建宏[1] 王茂林 袁国强[3] 潘亚文[1,4] DONG Qiang;XIE Qiqi;HU Jianhong;WANG Maolin;YUAN Guoqiang;PAN Yawen(Department of Neurosurgery,Second Hospital of Lanzhou University,Lanzhou 730030;Department of Orthopaedics,Second Hospital of Lanzhou University,Lanzhou 730030;Institute of Neurology,Lanzhou University,Lanzhou 730030,Gansu,China)

机构地区：[1]兰州大学第二医院神经外科,甘肃兰州730030 [2]兰州大学第二医院骨科,甘肃兰州730030 [3].兰州大学第二医院神经病学研究所,甘肃兰州730030 [4]兰州大学第二医院神经病学研究所,甘肃兰州730030

出　　处：《癌变．畸变．突变》2018年第6期457-462,共6页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金项目(81771297);甘肃省卫生行业科研计划项目(GSWSKY-2015-58);兰州市科技项目(2018-1-109)

摘　　要：目的:分析胶质瘤与正常组织表达差异的基因,筛选和胶质瘤预后相关的关键基因。方法:通过下载GEO(Gene ExpressionOmnibus)数据库GSE15824原始数据,利用R语言分析正常组织与胶质瘤组织中差异表达的基因,通过生物信息学分析工具(DAVID、String、Cytoscape、oncomine和GEPIA)对差异表达的基因进行生物学功能、蛋白互作网络(PPI)分析及筛选胶质瘤的预后标志物。结果:共筛选出648个差异表达的mRNAs。差异表达mRNAs的生物学功能主要富集于T细胞的激活,调节白细胞的黏附和细胞的迁移。KEGG信号通路分析显示差异基因主要富集于PI3K/Akt,MAPK和钙离子信号通路。FPR1在胶质瘤中明显高表达,其表达水平与胶质瘤的预后呈负相关(Log-rankP<0.01)。结论:通过基因芯片数据库的分析,FPR1在胶质瘤中高表达,且与患者生存预后相关,为进一步分子机制的研究提供了新思路。OBJECTIVE:To identify differentially expressed genes in gliomas which were related to their prognosis.METHODS:Raw data of the GEO(Gene Expression Omnibus)database GSE15824 involving normal and glioma tissues were analyzed using R language,and bioinformatics analysis tools(DAVID,String,Cytoscape,oncomine,and GEPIA).Differentially expressed genes were subjected to biological function,protein interaction analysis,and screening for prognostic markers of gliomas.RESULTS:A total of 648 differentially expressed mRNAs,including 471 up-regulated mRNAs and 177 down-regulated mRNAs were screened.Biological functions of these differential genes were mainly enriched in the activation of T cells,and regulation of adhesion of leukocytes and of cell migration.The KEGG signaling pathway was mainly enriched in PI3K/Akt,MAPK,and calcium signaling pathways.Formyl peptide receptor 1 was highly expressed in gliomas,and the expression level was negatively correlated with the prognosis of gliomas(Log-rank P<0.01).CONCLUSION:Through the analysis of gene expression microarray analysis,FPR1 was highly expressed in gliomas and was related to patients’survival.The observations provide new ideas for further molecular investigations.

关 键 词：胶质瘤 生物信息学 基因芯片 甲酰肽受体1 

分 类 号：R739.41[医药卫生—肿瘤]