白酒酿造废水制备微生物絮凝剂的研究  

Production of a microbial flocculant using Baijiu brewing wastewater

作　　者：周明罗 陈杰 游玲[2] 王涛[2] ZHOU Mingluo;CHEN Jie;YOU Ling;WANG Tao(College of Resources and Environmental Engineering,Yibin University,Yibin 644007,China;Key Lab of Fermentation Resource and Application of Institutes in Sichuan Higher Education,Yibin University,Yibin 644007,China)

机构地区：[1]宜宾学院资源与环境工程学院,四川宜宾644007 [2]宜宾学院发酵资源与应用四川省高校重点实验室,四川宜宾644007

出　　处：《中国酿造》2018年第11期86-90,共5页China Brewing

基　　金：四川省教育厅项目(13ZB0214);宜宾市科技局项目(2013SF025)

摘　　要：通过稀释倒平板法从活性污泥中筛选微生物絮凝剂产生菌,并以白酒酿造废水为培养基,在32℃条件下,研究废水化学需氧量(COD)、培养时间、pH值、外加氮、磷营养物等因素对微生物絮凝剂絮凝效果的影响。结果表明,从活性污泥中分离获得了产絮凝菌HN5,经16S r DNA基因序列比对分析鉴定为假中间苍白杆菌(Ochrobactrum pseudintermedium),培养产生的微生物絮凝剂有较好的絮凝效果。以白酒酿造废水作为替代培养基培养菌株,适宜的培养条件为:COD 5.0~6.9 g/L,培养时间60~72 h,pH值6~8,KH2PO42.0 g/L,所得微生物絮凝剂对高岭土悬浊液最高絮凝率为84.9%。用于处理生活污水时,向每100 m L污水中加入6.0 m L菌株培养液时,絮凝率可达86.8%,悬浮物去除率高达92.5%。利用白酒酿造废水作为微生物絮凝剂廉价培养基是可行的,既降低培养成本又能实现废液利用。The flocculant-producing strain was screened from activated sludge by pour plate method by using Baijiu(Chinese liquor)brewing wastewater as a medium.The effect of wastewater chemical oxygen demand(COD),culture time,pH,external nitrogen source,phosphorus source on flocculation effect of microbial flocculant were investigated at 32℃.The results showed that a flocculant-producing strain HN5 was screened,and identified as Ochrobactrum pseudintermedium based on 16S rDNA gene sequencing.The suitable culture conditions for microbial flocculants were as follows:wastewater COD 5.0-6.9 g/L,cultivation time 60-72 h,pH 6-8,KH2PO4 2.0 g/L.The microbial flocculants from Baijiu brewing wastewater had a good flocculation effect,and the highest flocculating rate to kaolin suspension was 84.9%.When 6.0 ml flocculant was added to 100 ml domestic sewage,the flocculation rate and the suspended solid removal rate were 86.8%and 92.5%,respectively.It was feasible to use Baijiu brewing wastewater as a cost-effective medium for microbial flocculants,which could not only reduce the cost of cultivation but also realize the reuse of wastewater.

关 键 词：微生物絮凝剂 白酒酿造废水 絮凝率 

分 类 号：X797[环境科学与工程—环境工程]