棉花黄萎病菌拮抗酵母菌筛选及鉴定  

Screening and Identification of Antagonistic Yeasts against Verticillium dahliae

作　　者：文雨婷 张媱 李杨[1] 孙燕飞[1] 雷勇辉[2] 王翀[3] WEN Yuting;ZHANG Yao;LI Yang;SUN Yanfei;LEI Yonghui;WANG Chong(College of Life Sciences,Shihezi University,Shihezi 832003,China;Agricultural College of Shihezi University,Shihezi 832003,China;Xinjiang Entry&Exit Inspection and Quarantine Bureau,Urumqi 830063,China)

机构地区：[1]石河子大学生命科学学院,新疆石河子832003 [2]石河子大学农学院,新疆石河子832003 [3]新疆出入境检验检疫局,新疆乌鲁木齐830063

出　　处：《河南农业科学》2018年第11期66-72,共7页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(31860003);石河子大学2014年高层次人才科研启动资金专项(RCZX201427);新疆特色植物基因资源研究与利用创新团队项目(2014CC005)

摘　　要：为获得对棉花黄萎病菌有拮抗效果的酵母菌株,以230株酵母菌为研究对象,通过平板对峙法和琼脂块法进行筛选,并利用形态特征、生理生化特性和26S r DNA序列分析对其进行鉴定。结果表明,筛选出的6株拮抗酵母菌(ZHB39、19-10、F3、S4B2-13、T1、MLY1)随着黄萎病菌孢子浓度的降低,其抑菌活性增加,当黄萎病菌孢子浓度为104cfu/mL时,拮抗酵母菌的抑菌圈直径最大,分别为12.50、13.83、16.33、25.17、21.83、11.17 mm,均具有较好的抑菌能力。6株拮抗酵母菌株分别被鉴定为美极梅奇酵母(Metschnikowia pulcherrima)、Kazachstania sp.、美极梅奇酵母、威克汉姆酵母(Wickerhamomyces anomalus)、葡萄汁有孢汉逊酵母(Hanseniaspora uvarum)、Barnettozyma californica。To obtain some yeasts that antagonized against Verticillium dahliae,230 yeast strains were screened by plate confrontation method and agar block method,and then identified through morphologic observation,physiological and biochemical character determination and 26S rDNA sequence analysis.The study showed that 6 antagonistic yeast strains were screened out,which were named ZHB39,19-10,F3,S4B2-13,T1 and MLY1.With the decrease of spore concentration of Verticillium dahliae,the 6 antagonistic yeast strains increased its antibacterial activity.And the diameters of bacteriostatic rings formed by antagonistic yeasts screened in this experiment reached the maximum when the spore concentration of Verticillium dahliae was 10 4 cfu/mL,which were respectively 12.50,13.83,16.33,25.17,21.83,11.17 mm.All of them performed good bacteriostatic effects.The result of identification showed that the 6 strains were respectively Metschnikowia pulcherrima,Kazachstania sp.,Metschnikowia pulcherrima,Wickerhamomyces anomalus,Hanseniaspora uvarum and Barnettozyma californica.

关 键 词：棉花 黄萎病菌 生物防治 拮抗 酵母菌 鉴定 

分 类 号：S435.621[农业科学—农业昆虫与害虫防治]