钠尿肽受体A(NPR-A)在小鼠角膜和晶状体发育过程中的表达  

Expression of natriuretic peptide receptor A in cornea and lens during the development of mouse

作　　者：李金菊 邓锦波[2] LI Jin-Ju;DENG Jin-Bo(Henan Luoyang Zhenggu Hospital,Luoyang 475001,Henan Province,China;Henan University Institute of Neurobiology,Kaifeng 475000,Henan Province,China)

机构地区：[1]河南省洛阳正骨医院(河南省骨科医院),河南省洛阳市475001 [2]河南大学神经生物研究所,河南省开封市475000

出　　处：《眼科新进展》2018年第12期1119-1122,1128,共5页Recent Advances in Ophthalmology

基　　金：国家自然科学基金面上项目(编号:30670688;30771140;31070952)~~

摘　　要：目的检测钠尿肽受体A(natriuretic peptide receptor A,NPR-A)在不同鼠龄小鼠角膜和晶状体内的表达,探讨其在小鼠眼发育过程中的作用。方法选用C57BL/6转基因小鼠,收集从E14到P90小鼠眼球标本120只,采用40 g·L^(-1)多聚甲醛灌注固定后,石蜡包埋切片,采用免疫组织化学方法对NPR-A在角膜和晶状体中的表达进行免疫荧光检测。结果在E16,NPR-A高表达于角膜上皮细胞中,并持续至成年;其在角膜基质层和内皮细胞的表达也始于E16,而在P14之后,NPR-A表达随鼠龄的增加逐渐减弱。此外,NPR-A在P0小鼠晶状体上皮细胞膜内被检测到,并持续高表达至成年。在E16,由晶状体后壁上皮细胞生成的初级纤维开始高表达NPR-A,但随着鼠龄的增长和纤维结构的改变而逐渐减弱,直到P90消失。结论 NPR-A可能参与了角膜和晶状体生长和发育,并且对维持角膜上皮细胞的增生和修复以及晶状体的通透性具有重要作用。Objective To investigate the expression of natriuretic peptide receptor A(NPR-A)in the mouse cornea and lens and to understand the NPR-A’s functions during the mouse development.Methods Total 120 cases of E14 to P90 C57BL/6 transgenic mouse eye specimens were collected and fixed by 40 g·L^-1 paraformaldehyde and made paraffin embedded slices,Then,immunohistochemistries of NPR-A were carried out.Results At E16,NPR-A was highly expressed in embryonic corneal epithelial cells,and it continued to express until adulthood.However,P14 afterward,its expression in the corneal stroma and endothelium layer was decreased gradually with age increasing.On the other hand,NPR-A could be found in developing lens as well.For instance,at P0,NPR-A began to appear in the frontal epithelium of lens,and continued to express highly until adulthood.NPR-A could also be expressed highly in lens fibroblasts which were developed from posterior epithelium.With development,its expression decreased gradually,and disappeared in lens fibers totally after P90.Conclusion NPR-A may be involved in the development of cornea and lens.It plays an important role in corneal epithelial cell proliferation and lens repair.On the other hand,the transparency of lens probably is regulated by NPR-A as well.

关 键 词：角膜和晶状体 发育 钠尿肽受体A 免疫组织化学 

分 类 号：R772[医药卫生—眼科]