PI3K/Akt和Wnt/β-catenin信号通路在大鼠脑缺血再灌注损伤中的相互作用  

Interaction between PI3K/Akt and Wnt/β-catenin signaling pathway in cerebral ischemia-reperfusion rats

作　　者：邱月 邢雪松[1] 吕威力[2] QIU Yue;XING Xuesong;LV Weili(Department of Anatomy,Shenyang 110034,China)

机构地区：[1]沈阳医学院解剖学教研室 [2]沈阳医学院病理学教研室,辽宁沈阳110034

出　　处：《中风与神经疾病杂志》2018年第11期978-982,共5页Journal of Apoplexy and Nervous Diseases

基　　金：辽宁省科技厅科学技术计划资助项目(201602729);沈阳市科技计划项目(18-013-0-50);辽宁省重点研发计划指导计划项目(2017225063)

摘　　要：目的探究PI3K/Akt信号通路在大鼠脑缺血再灌注损伤中对Wnt/β-catenin信号通路的影响。方法采用longa改良法建立局灶性脑缺血再灌注模型,将78只健康雄性SD大鼠随机分为4组:假手术组(S组)、脑缺血再灌注组(I/R组)、脑缺血再灌注+bFGF后处理组(b FGF组)、脑缺血再灌注+bFGF后处理+LY294002(LY组)。应用HE染色检测大脑皮质组织形态变化,TUNEL法检测细胞凋亡情况。应用免疫组织化学染色法、原位杂交法分别检测在各组大鼠脑皮质在不同再灌注时间点P-Akt、GSK-3βmRNA表达变化情况。结果相较于假手术组(S组),I/R组和LY组凋亡细胞数和P-Akt、GSK-3βmRNA表达明显增高(P <0. 05); b FGF处理组凋亡细胞数和GSK-3βmRNA表达减少,但P-Akt表达量较I/R组和LY组显著增高(P <0. 05)。结论在脑缺血再灌注损伤中,PI3K/Akt信号通路可以通过调控GSK-3β进而影响Wnt/β-catenin信号通路,这为深入研究PI3K/Akt和Wnt/β-catenin信号通路在缺血性脑损伤中的协同作用机制提供重要线索。Objective To investigate the effect of PI3K/Akt signaling pathway on Wnt/β-catenin signaling pathway in rats with cerebral ischemia-reperfusion injury.Methods Ninety-six healthy male SD rats were randomly divided into 4 groups:sham operation group(s group),cerebral ischemia and reperfusion group(I group),cerebral ischemia reperfusion+bFGF postconditioning group(bFGF group),cerebral ischemia reperfusion+bFGF postconditioning+ly294200(LY group).HE staining and TUNEL method were used to detect cortical tissue morphological changes and apoptosis.The expression of p-Akt and GSK-3βmRNA expression in cortex of each group at different reperfusion time points were detected by immunohistochemistry and in situ hybridization.Results The number of apoptotic cells and the expressions of p-Akt and GSK-3βmRNA in I group and LY group were higher than those in group(P<0.05).The number of apoptotic cells and the expression of GSK-3βmRNA in bFGF group decreased,and the expression of p-Akt was significantly higher than that in I group and LY group(P<0.0 Conclusion 5).Conclusion PI3K/Akt signaling pathway can regulate Wnt/β-catenin signaling pathway through GSK-3βin cerebral ischemia-reperfusion injury,which provides important clues to further study the synergistic mechanism of PI3K/Akt and Wnt/β-catenin signaling pathway in ischemic brain injury.

关 键 词：脑缺血 Akt GSK-3Β BFGF LY294002 皮质 

分 类 号：R743[医药卫生—神经病学与精神病学]