不同剂量率高能X射线对宫颈癌HeLa细胞生物学效应的影响  

Effect of High Energy X-rays at Different Dose Rates on Biological Effects of Cervical Cancer HeLa Cells

作　　者：李芷茹 李超[1] 曾守群[1] 何朗 黄建鸣[2] 郎锦义[2] LI Zhiru;LI Chao;ZENG Shouqun;HE Lang;HUANG Jianming;LANG Jinyi(Department of Oncology,The Fifth People’s Hospital of Chengdu,Chengdu 611130,China;Department of Radiation Oncology,Sichuan Cancer Hospital,Chengdu 610041,China)

机构地区：[1]成都市第五人民医院肿瘤科,成都611130 [2]四川省肿瘤医院放疗中心,成都610041

出　　处：《肿瘤防治研究》2018年第11期870-873,共4页Cancer Research on Prevention and Treatment

摘　　要：目的观察高能X射线不同剂量率照射对宫颈癌HeLa细胞株克隆形成率、存活率及放射生物学参数的影响。方法将指数生长期的人宫颈癌He La细胞分别采用2、6、10 Gy/min三个剂量率梯度单次照射,照射剂量分别为0、1、2、4、6、8 Gy。照射后置培养箱中继续培养12 d后0.5%结晶紫染色固定,计数大于50个细胞的克隆数。采用GraphPad Prism 5.0软件,根据单靶多击数学模型和线性二次方程(LQ)模型拟合细胞存活曲线,求出三种不同剂量率下HeLa细胞相关放射生物学参数。采用SPSS19.0软件对数据进行统计学分析,组间比较采用随机区组方差分析。结果 (1)HeLa细胞在三种剂量率照射后,对克隆形成率和存活率的影响差异均有统计学意义(P=0.03和0.04)。(2)三种高能X线不同剂量率照射下平均α/β值分别为3.28 Gy、3.35 Gy、3.93 Gy,SF2分别为0.79、0.78、0.75,三种剂量率对生物学参数α/β、D0、Dq、SF2差异无统计学意义(P>0.05)。结论高能X射线三种不同剂量率照射He La细胞后,随着高能X射线剂量率增大,He La细胞存活率趋势逐渐下降,生物学效应逐渐增加。Objective To investigate the effects of high-energy X-rays irradiation at different dose rates on the plating efficiency,survival fraction and radiobiological parameters of cervical cancer HeLa cell line.Methods Human cervical cancer Hela cell line in exponential growth phase were treated with 0,1,2,4,6,8Gy at 2,6,10Gy/min dose rates.After irradiation for 12 days in the incubator,0.5%crystal violet staining was fixed,and the number of clones larger than 50 cells was counted.The data was analyzed and dosesurvival curves were plotted following the standard linear-quadratic model using GraphPad Prism 5.0 software to get the values of radiobiological parameters.Differences of survival fraction with different dose rates were tested with analysis of variance by SPSS19.0 software.Results After 2,6,10 Gy/min of high-energy X-rays irradiation,the differences between the plating efficiency and survival fraction of HeLa cells were statistically significant(P=0.03 and 0.04).The averageα/βvalues were 3.28Gy,3.35Gy,3.93Gy,SF2 were 0.79,0.78,0.75.Three dose rates showed no statistically significant difference in the biological parameters,α/β,D0,Dq or SF2(P>0.05).Conclusion With the increasing dose rate of high-energy X-rays(2,6,10Gy/min),the survival rate of HeLa cells gradually decreases while the biological effect gradually increases.

关 键 词：X射线 宫颈癌 剂量率 生物学效应 

分 类 号：R737.33[医药卫生—肿瘤] R730.55[医药卫生—临床医学]