三种噻唑烷二酮类药物对宫颈癌细胞增殖的影响  

Effect of thiazolidinedione drugs on cervical cancer cell proliferation

作　　者：屈秋慧 韩红芳[2] 吴胜军[1] 张小丽 李艳川 QU Qiu-hui;HAN Hong-fang;WU Sheng-jun(Department of Obstetrics and Gynecology,The Second Affiliated Hospital of Xi'an Medical College,Xi'an Shaanxi 710038,China;Department of Obstetrics and Gynecology,The Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an Shaanxi 710004,China)

机构地区：[1]西安医学院第二附属医院妇产科,陕西西安710038 [2]西安交通大学第二附属医院妇产科,陕西西安710004 [3]甘肃省第二人民医院妇产科,甘肃兰州730000 [4]陕西省人民医院妇产科,陕西西安710068

出　　处：《临床和实验医学杂志》2018年第23期2490-2494,共5页Journal of Clinical and Experimental Medicine

基　　金：甘肃省科技计划项目(编号:2011y0075)

摘　　要：目的探讨吡格列酮、罗格列酮以及环格列酮三种TZDs对宫颈癌细胞(CCCs)增殖的影响以及潜在的分子机制。方法分别以0μM、2. 5μM、5μM以及10μM浓度的吡格列酮、罗格列酮以及环格列酮刺激人宫颈癌细胞系HeLa(HPV 18+),CCK-8方法检测细胞增殖,油红O染色分光光度计法检测脂肪累积,实时定量PCR检测PPARγmRNA的表达。结果 2. 5μM和5μM的吡格列酮、5μM的环格列酮、以及10μM的TZDs在刺激第5天时均可显著抑制CCCs的增殖(P <0. 05)。吡格列酮与20μM顺铂或者100μM的5-氟尿嘧啶联合刺激比单独10μM吡格列酮刺激更能显著抑制CCCs的增殖(P <0. 05)。10μM的TZDs均可显著增加CCCs中脂质的累积(P <0. 05)。2. 5μM、5μM和10μM的TZDs刺激CCCs 5 d,均可显著增加PPARγmRNA的表达(P <0. 05)。结论三种TZDs均可抑制CCCs的增殖,其机制可能与TZDs通过促进PPARγ的表达进而增加癌细胞中脂质的累积有关。Objective The effect of three TZDs,namely pioglitazone(pio),rosiglitazone(ros)and ciglitazone(cig),on the proliferation of cervical cancer cells(CCCs)was studied and underlying mechanisms probed.Methods Human CCCs line HeLa(HPV 18+)was stimulated with pio,ros and cig at concentrations of 0μM,2.5μM,5μM and 10μM respectively.Cell proliferation was detected by CCK-8 method.Fat accumulation was analyzed by spectrophotometer after oil red O staining.Expression of PPAR mRNA was detected by real-time quantitative PCR.Results 2.5μM and 5μM pio,5μM cig,and 10μM TZDs significantly inhibited the proliferation of CCCs on 5 d(P<0.05).The combination of pio with 20μM cis-platinum or 100μM 5-fluorouracil inhibited the proliferation of CCCs more than 10 M pio alone(P<0.05).10μM TZDs significantly increased lipid accumulation in CCCs(P<0.05).2.5μM,5μM and 10μM TZDs significantly increased the expression of PPARγmRNA in CCCs(P<0.05).Conclusion The three TZDs can inhibit the proliferation of CCCs.TZDs might promote the expression of PPARγthus the accumulation of lipids in cancer cells.

关 键 词：宫颈癌 细胞增殖 吡格列酮 罗格列酮 环格列酮 

分 类 号：R737.33[医药卫生—肿瘤]