酵母硒和苜草素联用对产蛋后期蛋鸡蛋品质、抗氧化能力和胆固醇代谢的影响  

Effects of Combination of Selenium Yeast and Polysavone on Egg Quality,Antioxidant Ability and Cholesterol Metabolism of Hens during Late Period of Laying

作　　者：杨玉[1] 孙煜[1] 孙宝盛[1] 卢营杰 李建慧[1] 张俊珍[1] YANG Yu;SUN Yu;SUN Baosheng;LU Yingjie;LI Jianhui;ZHANG Junzhen(College of Animal Science and Technology,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学动物科技学院,太谷030801

出　　处：《动物营养学报》2018年第10期4095-4104,共10页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：山西省科学技术发展计划项目(201603D221033-1);山西省现代农业鸡产业技术体系(20171102);山西功能农业共性关键技术研究与示范(201703D211001);晋中市科技重点研发计划(农业)(201803D01100006)

摘　　要：本试验旨在研究不同水平酵母硒和苜草素联用对产蛋后期蛋鸡蛋品质、抗氧化能力和胆固醇代谢的影响。试验选取756只69周龄海兰褐蛋鸡,随机分为7组,每组6个重复,每个重复18只。对照组饲喂基础饲粮,试验组分别在基础饲粮中添加0.2 mg/kg酵母硒+500 mg/kg苜草素、0.2 mg/kg酵母硒+1 000 mg/kg苜草素、0.6 mg/kg酵母硒+500 mg/kg苜草素、0.6 mg/kg酵母硒+1 000 mg/kg苜草素、1.0 mg/kg酵母硒+500 mg/kg苜草素和1.0 mg/kg酵母硒+1 000 mg/kg苜草素。试验预试期2周,正试期8周。结果表明:1)饲粮添加酵母硒和苜草素可显著提高蛋鸡产蛋率(P<0.05),显著降低料蛋比(P<0.05),有提高鸡蛋蛋壳强度、蛋白高度和哈氏单位的趋势。2)饲粮添加酵母硒和苜草素极显著提高蛋鸡血浆谷胱甘肽过氧化物酶(GSH-Px)和总超氧化物歧化酶活性、肝脏GSH-Px活性以及肝脏GSH-Px1、硫氧还蛋白还原酶1 mRNA表达量(P<0.01);极显著降低蛋鸡肝脏甘油三酯含量和3-羟基-3-甲基戊二酸单酰辅酶A还原酶mRNA表达量(P<0.01),极显著提高肝脏胆固醇7α-羟化酶和固醇调节元件结合蛋白-1c mRNA表达量(P<0.01);极显著提高蛋黄硒含量(P<0.01)。3)添加0.6 mg/kg酵母硒+1 000 mg/kg苜草素可极显著提高蛋鸡血浆总抗氧化能力(P<0.01),极显著降低蛋鸡血浆丙二醛含量(P<0.01),极显著降低蛋黄胆固醇和甘油三酯含量以及肝脏胆固醇含量(P<0.01)。综上,饲粮中添加酵母硒和苜草素可提高产蛋后期蛋鸡生产性能、蛋品质、抗氧化能力及胆固醇代谢功能,以0.6 mg/kg酵母硒+1 000 mg/kg苜草素组合效果最佳。This experiment was conducted to research the effects of combination of different levels of selenium yeast and polysavone on egg quality,antioxidant ability and cholesterol metabolism of hens during late period of laying.Seven hundred and fifty-six 69-week-old Hyline Brown hens were randomly allocated into 7 groups with 6 replicates of 18 hens for each replication.Hens in the control group were fed a basal diet,and those in experimental groups were fed the basal diet supplemented with 0.2 mg/kg selenium yeast+500 mg/kg polysavone,0.2 mg/kg selenium yeast+1 000 mg/kg polysavone,0.6 mg/kg selenium yeast+500 mg/kg polysavone,0.6 mg/kg selenium yeast+1 000 mg/kg polysavone,1.0 mg/kg selenium yeast+500 mg/kg polysavone and 1.0 mg/kg selenium yeast+1 000 mg/kg polysavone,respectively.The preliminary experiment was lasted for 2 weeks and the formal experiment lasted for 8 weeks.The results showed as follows:1)dietary selenium yeast and polysavone significantly increased the laying rate(P<0.05),significantly reduced the ratio of feed to egg(P<0.05),and tended to improve the eggshell thickness,albumen height and Haugh unit of laying hens.2)Dietary selenium yeast and polysavone extremely significantly increased the activities of plasma glutathione peroxidase(GSH-Px)and total superoxide dismutase,GSH-Px activity in liver,mRNA expression levels of GSH-Px1 and thioredoxin reductase in liver of laying hens(P<0.01).Dietary selenium yeast and polysavone extremely significantly reduced the triglyceride content and the 3-hydroxyl-3-methyl-glutarate monoacyl coenzyme A reductase mRNA expression level in liver of laying hens(P<0.01),extremely significantly increased the mRNA expression levels of cholesterol 7α-hydroxylase and sterol regulatory element binding protein in liver of laying hens(P<0.01),and extremely significantly increased the selenium content in yolk(P<0.01).3)The combination of 0.6 mg/kg selenium yeast+1 000 mg/kg polysavone could extremely significantly increase plasma total antioxidant capacity(P<0.01),extremely si

关 键 词：酵母硒 苜草素 蛋鸡 蛋品质 抗氧化能力 胆固醇代谢 

分 类 号：S816[农业科学—饲料科学]