氧化锆陶瓷托槽对变形链球菌附着的影响  

Study on adhesion of Streptococcus mutans to zirconia ceramic brackets

作　　者：李文艳 李晨军[2] 羊书勇[2] 何勇[2] Wen-yan Li;Chen-jun Li;Shu-yong Yang;Yong He(College of Stomatology,Southwest Medical University,Luzhou,Sichuan 646000,China;Hospital of Stomatology,General Hospital of Chengdu Military Region,Chengdu,Sichuan 610021,China)

机构地区：[1]西南医科大学口腔医学院,四川泸州646000 [2]成都军区总医院附属口腔医院,四川成都610021

出　　处：《中国现代医学杂志》2018年第35期74-77,共4页China Journal of Modern Medicine

摘　　要：目的评估氧化锆陶瓷托槽对变形链球菌附着性的影响。方法将两种托槽模仿口内环境在含有唾液的脑心浸出液肉汤培养基中培养72 h后,利用实时荧光定量聚合酶链式反应的TaqMan探针法定量分析样本中变形链球菌和总细菌的数量,计算出变形链球菌的构成比。最后对两种托槽样本的细菌构成比进行对比分析。结果两种托槽表面的变形链球菌构成比比较,差异无统计学意义(P>0.05),两种托槽表面的变形链球菌数目和总细菌数目比较,差异无统计学意义(P>0.05)。结论氧化锆陶瓷托槽的变形链球菌及总细菌的附着水平与氧化铝陶瓷托槽无明显差异,可供临床进一步研究。Objective To evaluate the impact of zirconia ceramic brackets on the adhesion of Streptococcus mutans.Methods In the experiment,18 brackets were collected,including 9 zirconia ceramic brackets in the observation group and 9 alumina ceramic brackets in the control group.After the brackets in both groups were cultured in the brain heart infusion broth with saliva for 72 hours,TaqMan probe method of qRT-PCR was applied to analyze the amount of Streptococcus mutans and total bacteria,thus the composition ratio of Streptococcus mutans was calculated.A comparative study in the composition ratio of bacteria was carried out between the two groups.Results There was no statistical difference in composition ratio of Streptococcus mutans to total bacteria between the two groups(P>0.05),either in the amount of Streptococcus mutans or total bacteria adhered to the brackets between the two groups(P>0.05).Conclusions The adhesion of Streptococcus mutans and total bacteria to zirconia ceramic brackets is of no obvious difference from that to alumina ceramic brackets,which needs further clinical research.

关 键 词：细菌 正畸托架 牙瓷料 聚合酶链反应 

分 类 号：R783.5[医药卫生—口腔医学]