芒果炭疽病菌侵染过程中致病基因的表达分析  

Expression Analysis of Pathogenic Genes During the Infection of Colletotrichum gloeosporioides to Mango

作　　者：苏初连 康浩 梅志栋[3] 杨石有 刘晓妹 蒲金基 张贺[2] SU Chu-lian;KANG Hao;MEI Zhi-dong;YANG Shi-you;LIU Xiao-mei;PU Jin-ji;ZHANG He(Institute of Tropical Agriculture and Forestry,Hainan University,Haikou 570228;Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Key Laboratory of Integrated Pest Management on Tropical Crops,Ministry of Agriculture,Haikou 571101;Chengmai County Agriculture Bureau,Chengmai 571900)

机构地区：[1]海南大学热带农林学院,海口570228 [2]中国热带农业科学院环境与植物保护研究所,海口571101 [3]澄迈县农业局,澄迈571900

出　　处：《生物技术通报》2018年第10期182-186,共5页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31460455);中国热带农业科学院基本科研业务费专项资金(1630042017019;1630042017030);海南省重大科技计划项目(ZDKJ2017003);国家重点研发计划(2017YFD0202107-3)

摘　　要：为研究芒果胶孢炭疽菌在侵染过程中致病相关基因的差异表达。采用实时荧光定量PCR的方法,分析病菌侵染芒果叶片和果实过程中PL、PG等11个基因的表达量变化。研究发现,病原菌侵染叶片时,PG和PL基因均持续高效表达,SIN3P基因表达较低,其余基因在侵染6 h时表达量较高,随后下降;病原菌侵染果实时,PL、PG、SDH、ECH等基因高效表达,其余基因则有升有降。说明致病基因在胶孢炭疽菌侵染芒果不同组织时表达有差异,在侵染的不同时段也不同程度地发挥功能。This work aims to study the differential expressions of pathogenicity-related genes during the process of infecting mango by Colletotrichum gloeosporioides.Quantitative real-time PCR was utilized to analyze the expression variations of 11 genes including PL and PG during pathogen infecting mango leaves and fruits.The results showed that the PG and PL genes were highly expressed continuously and the expression of SIN3P gene was low,while the other genes expressed high at 6 h after infection and then decreased during the process of C.gloeosporioides infection to mango leaves.PL,PG,SDH,ECH and other genes highly expressed when the pathogen infected the fruit,while the rest of the genes either increased or decreased.These results indicate that the pathogenic genes express differentially while C.gloeosporioides infects different tissues,and play different roles in the different infection period at varied level.

关 键 词：胶孢炭疽菌 致病基因 表达分析 

分 类 号：S436.67[农业科学—农业昆虫与害虫防治]