去酰基化ghrelin抑制上皮性卵巢癌细胞的增殖  

Des-Acyl Ghrelin Inhibits the Proliferation of Ovarian Epithelial Carcinoma Cells

作　　者：窦磊[1] 庞晓燕[1] 李奇 尚海[2] 张颐[1] DOU Lei;PANG Xiaoyan;LI Qi;SHANG Hai;ZHANG Yi(Department of Gynecology,The First Hospital,China Medical University,Shenyang 110001,China;Department of Hepatobiliary Surgery,Liaoning Tumor Hospital&Institute,Shenyang 110042,China)

机构地区：[1]中国医科大学附属第一医院妇科,沈阳110001 [2]辽宁省肿瘤医院肝胆胰外科,沈阳110042

出　　处：《中国医科大学学报》2018年第12期1102-1106,共5页Journal of China Medical University

基　　金：沈阳市科技计划(17-231-1-54)

摘　　要：目的探讨去酰基化ghrelin (DAG)对上皮性卵巢癌细胞增殖的作用及其相关分子机制。方法培养上皮性卵巢癌细胞SKOV3,以DAG处理,并予以激动剂干预哺乳动物雷帕霉素靶蛋白(m TOR)和经典Wnt信号途径,采用CCK-8法测定细胞增殖。结果DAG呈剂量依赖性抑制卵巢癌SKOV3细胞的增殖。DAG抑制m TOR信号途径,抑制经典Wnt信号转录活性以及下游靶基因的mRNA水平,mTOR和Wnt信号激活可在一定程度上翻转DAG对SKOV3细胞增殖的抑制作用。结论 DAG通过抑制m TOR和经典Wnt信号通路发挥其抑制卵巢癌细胞增殖的作用,为卵巢癌防治提供新思路。Objective To assess the effect of des-acyl ghrelin(DAG)on the proliferation of ovarian epithelial carcinoma cells and the related mechanism.Methods SKOV3 ovarian epithelial carcinoma cells were cultured and treated with DAG,with or without mammalian target of rapamycin(mTOR)and Wnt signaling pathway activator.Cell proliferation was measured by the CCK-8 assay.Results DAG inhibited the proliferation and growth of SKOV3 cells in a dose-dependent manner.DAG exposure induced the inhibition of the mTOR and Wnt signaling pathways.Activation of these two pathways blocked the inhibition of SKOV3 cell proliferation by DAG.Conclusion DAG inhibits the proliferation of SKOV3 ovarian epithelial carcinoma cells through the mTOR and Wnt signaling pathways,and may contribute to prevention and therapy of ovarian epithelial carcinoma.

关 键 词：去酰基化ghrelin 上皮性卵巢癌 增殖 mTOR信号途径 WNT信号途径 

分 类 号：R737.31[医药卫生—肿瘤]