雾化吸入灭活草分枝杆菌对脓毒症急性肺损伤大鼠Toll样受体4及核因子-kappa B表达的影响  

Effects of Inhaled Inactivated-Mycobacterium Phlei on Expression of Toll-like Receptor 4 and Nuclear Factor kappa B in Septic Rats with Acute Lung Injury

作　　者：齐心欣[1] 顾忠民[1] 姚雯 QI Xinxin;GU Zhongmin;YAO Wen(Department of Intensive Care Unit, Fuzhou No.2 Hospital Affiliated to Xiamen University,Fuzhou City Fujian Province 350007)

机构地区：[1]厦门大学附属福州市第二医院重症医学科,福建省福州市350007

出　　处：《医学理论与实践》2018年第23期3473-3474,3480,共3页The Journal of Medical Theory and Practice

基　　金：2016福州市卫生计生系统科技计划项目(No.1;2016-S-wt2)

摘　　要：目的:分析脓毒症急性肺损伤大鼠以雾化吸入灭活草分枝杆菌治疗后,其Toll样受体4(TLR4)、核因子-kappa B(NF-κB)水平表达情况。方法:选取健康清洁级Wistar大鼠48只随机分为对照组和观察组,每组24只,其中对照组5ml生理盐水雾化吸入+盲肠结扎穿刺行脓毒症急性肺损伤造模,观察组大鼠雾化吸入草分枝杆菌F. U. 36注射液后造模,于术后1d、2d取大鼠肺组织以RT-PCR法对TLR4、NF-κB进行检测,比较两组大鼠不同时间段内TLR4、NF-κB水平变化差异。结果:观察组术后1d、2d TLR4相对表达量高于对照组(P <0. 05);术后1d、2d观察组NF-κB相对表达量低于对照组(P <0. 05)。结论:脓毒症急性肺损伤时大鼠TLR4、NF-κB表达增强,雾化吸入灭活草分枝杆菌后NF-κB表达降低,提示此药通过影响炎症信号通路蛋白表达,从而减轻患者病情。Objective:To analyze the expression of Toll like receptor4(TLR4)and nuclear factor-kappa B(NF-kappa B)in rats with acute lung injury of sepsis after inhalation and inactivation of Mycobacterium Mycobacterium.Methods:48healthy and clean Wistar rats were randomly divided into control group and observation group,with24rats in each group.The control group was treated with5ml physiological saline inhalation and cecum ligation for acute lung injury of sepsis.The rats in the observation group were nebulized by Mycobacterium Mycobacterium F.U.36injection,and the rat lung tissue was taken on1day and2days after the operation.TLR4and NF-kappa B were detected by RT-PCR method.The difference of TLR4and NF-kappa B levels in two groups of rats at different time intervals were compared.Results:The relative expression of TLR4in the observation group was higher than that in the control group on the1day and the 2 days after operation(P<0.05);the relative expression of NF-kappa B in the observation group was lower than that in the control group after1day and2days after operation(P<0.05).Conclusion:The expression of TLR4and NF-kappa B in rats with acute lung injury was enhanced.The expression of NF-kappa B was reduced after atomization inhalation and inactivation of Mycobacterium Mycobacterium,suggesting that the drug could reduce the patient’s condition by affecting the expression of inflammatory signaling pathway protein.

关 键 词：NF-ΚB 灭活草分枝杆菌 急性肺损伤 TLR4 脓毒症 

分 类 号：R-33[医药卫生]