星点设计-效应面法优化鹿角多肽酶解工艺  

Optimization of enzymatic hydrolysis of antlers polypeptide by central composite design-response surface methodology

作　　者：王平 张会敏[2] 李刚[1] 孙铁锋[2] 秦文[3] 刘凡杰 WANG Ping;ZHANG Hui-min;LI Gang;SUN Tie-feng;QIN Wen;LIU Fan-jie(State Key Laboratory of Precision Measurement Technology and Instruments, Tianjin University, Tianjin 300072;Shandong Academy of Chinese Medicine, Jinan 250014;School Hospital of Shandong University, Jinan 250012;Shandong Medicinal Biotechnology Center, Jinan 250062)

机构地区：[1]天津大学精密测试技术及仪器国家重点实验室,天津300072 [2]山东省中医药研究院,山东济南250014 [3]山东大学校医院,山东济南250012 [4]山东省医药生物技术研究中心,山东济南250062

出　　处：《山东科学》2018年第6期1-10,共10页Shandong Science

基　　金：国家自然科学基金(81273776);山东省自然科学基金(NO.ZR2015HM061,ZR2014HQ046);山东省重点研发计划(2016GSF202042);山东省中医药科技发展计划(2013Z002-5);泰山学者岗位(ts201511074);山东省五级师承计划

摘　　要：采用星点设计-效应面法优化鹿角多肽酶解工艺。煎煮法提取鹿角胶后经胃蛋白酶水解得到鹿角多肽,采用单因素法初步考察底物浓度、酶用量、温度、p H、酶解时间等因素对多肽峰面积及含量的影响;再以底物浓度、酶用量、温度为自变量,双缩脲法测得多肽含量及HPLC法测得峰面积总和为因变量进行星点设计试验,确定最佳酶解工艺,并进行验证。结果表明,星点设计-效应面法得到的最佳酶解条件为酶用量1. 8%、底物浓度0. 08 mg/m L、温度40℃。该方法简便、可行、稳定,预测性良好。The optimization of enzymatic hydrolysis of antlers polypeptide was conducted by central composite design-response surface methodology.The antlers polypeptide was obtained by pepsin hydrolysis antlers collagen which was decocted extract from antlers.The effects of substrate concentration,enzyme dosage,temperature,pH and enzymolysis time on the peak areas and polypeptide content of peptides were investigated by single factor method.Then,substrate concentration,enzyme dosage and temperature were taken as the independent variables.Meanwhile,the polypeptide content of peptides and polypeptide peak areas were taken as the dependent variables for the central composite design-response surface test which could determine the best enzymatic hydrolysis process.Finally,the experiment was verified.The results indicated that the best processing conditions were:enzyme loading was1.8%,substrate concentration was0.08mg/mL and the temperature was40℃.The method was simple,feasible,stable and predictive.

关 键 词：鹿角多肽 酶解 星点设计-效应面法 

分 类 号：R284.2[医药卫生—中药学]