MS-222和丁香酚在大口黑鲈幼鱼模拟运输中的麻醉效果  

Anesthetic effects of MS-222 and eugenol in simulated transportation of juvenile largemouth bass(Micropterus salmoides)

作　　者：王文豪 董宏标[1] 孙永旭 曹明 段亚飞[1] 李华[1] 刘青松[1] 张家松[1,4] WANG Wenhao;DONG Hongbiao;SUN Yongxu;CAO Ming;DUAN Yafei;LI Hua;LIU Qingsong;ZHANG Jiasong(Key Laboratory of South China Sea Fishery Resources Exploitation&Utilization,Ministry of Agriculture and Rural Affairs;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China;College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;Guangdong Provincial Fishery Germplasm Conservation Center,Guangzhou 511453,China;Shenzhen Base of South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shenzhen 518121,China)

机构地区：[1]中国水产科学研究院南海水产研究所,农业农村部南海渔业资源开发利用重点实验室,广东广州510300 [2]上海海洋大学水产与生命学院,上海201306 [3]广东省渔业种质保护中心,广东广州511453 [4]中国水产科学研究院南海水产研究所深圳试验基地,广东深圳518121

出　　处：《南方水产科学》2018年第6期52-58,共7页South China Fisheries Science

基　　金：中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助(2017YB15,2016TS07);广东省自然科学基金项目(2015A030310393,2017A030313147);中国-东盟海上合作基金项目“中国-东盟现代海洋渔业技术合作及产业化开发示范”

摘　　要：为确定在实验条件下大口黑鲈(Micropterus salmoides)幼鱼适合的麻醉方式与麻醉剂量,研究比较了7种不同质量浓度的MS-222 (40 mg·L^(–1)、50 mg·L^(–1)、60 mg·L^(–1)、70 mg·L^(–1)、80 mg·L^(–1)、90 mg·L^(–1)、100 mg·L^(–1))和丁香酚(8 mg·L^(–1)、10 mg·L^(–1)、12 mg·L^(–1)、14 mg·L^(–1)、16 mg·L^(–1)、18 mg·L^(–1)、20 mg·L^(–1)) 2种常见渔用麻醉剂对体质量为(130±10) g的大口黑鲈幼鱼的静水麻醉效应。静水麻醉实验结果表明:1)随着2种麻醉剂浓度升高,幼鱼入麻时间缩短,复苏时间延长;2)在实验条件下,MS-222与丁香酚的最适麻醉质量浓度分别为70 mg·L^(–1)和16 mg·L^(–1),最适运输质量浓度分别为50 mg·L^(–1)和10 mg·L^(–1)。使用最适运输浓度的MS-222和丁香酚对幼鱼进行10 h模拟运输,观察麻醉效应和分析血清生化指标发现:1)麻醉组运输成活率和复苏后24 h成活率均为100%,显著高于无麻醉运输组的80%和60%(P<0.05);2)较之无麻醉运输组和静水对照组,麻醉运输组的丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)水平显著升高(P<0.05)。在麻醉运输组中,MS-222麻醉组的ALT和AST水平显著高于丁香酚麻醉组(P<0.05)。To determine the appropriate anesthetic method and dose for juvenile Micropterus salmoides in laboratory,we compared the hydrostatic anesthesia effects of two common fish anesthetics(MS-222 and eugenol)on the juveniles with different con-centrations(40 mg·L^–1,50 mg·L^–1,60 mg·L^–1,70 mg·L^–1,80 mg·L^–1,90 mg·L^–1,100 mg·L^–1 and 8 mg·L^–1,10 mg·L^–1,12 mg·L^–1,14 mg·L^–1,16 mg·L^–1,18 mg·L^–1,20 mg·L^–1).The results show:1.With increasing concentrations of the two anesthetics,the anesthetic time shortened but the recovery time prolonged.2.The optimal anesthetic concentrations of MS-222 and eugenol were 70 mg·L^–1 and 16 mg·L^–1,respectively,while the optimal anesthetic transportation concentrations of the two anesthetics were 50 mg·L^–1 and 10 mg·L^–1,respectively.Then we carried out an experiment of 10-hour anesthesia transport under the optimal anesthetic concentrations of MS-222 and eugenol,and the survival rate during the transportation and after 24 h of recovery for the anesthesia group were both 100%,significantly higher than those for the non-anesthetic transport group(P<0.05).The alanine aminotransferase(ALT)and aspartate aminotransferase(AST)levels increased significantly in the anesthesia transport group compared with those in the non-anesthetic transport group and hydrostatic control group(P<0.05).The ALT and AST levels in the MS-222 anesthesia group were significantly higher than those in the eugenol anesthesia group(P<0.05).

关 键 词：大口黑鲈 麻醉运输 MS-222 丁香酚 

分 类 号：S981.13[农业科学—捕捞与储运]