AXL激酶抑制剂对鼻咽癌细胞增殖、迁移和侵袭的影响  

Effects of AXL kinase inhibitor on proliferation,migration,and invasion of nasopharyngeal carcinoma cells

作　　者：程健 张磊[2] 秦克旺[2] CHENG Jian;ZHANG Lei;QIN Kewang(The Second Clinical Medical School of Southern Medical University, Guangzhou 510317, China)

机构地区：[1]南方医科大学第二临床医学院,广州510317 [2]广东省第二人民医院

出　　处：《山东医药》2018年第45期57-60,共4页Shandong Medical Journal

摘　　要：目的探讨AXL激酶抑制剂TP0903对鼻咽癌细胞增殖、迁移和侵袭的影响。方法取对数生长期鼻咽癌5-8F、SUNE2细胞,分别分为对照组和实验组。对照组未加入TP0903。实验组中加入0.5、1.0、2.0、4.0μmol/L TP0903作用于鼻咽癌细胞5-8F,0.125、0.25、0.5μmol/L TP0903作用于鼻咽癌细胞SUNE2。培养72 h后采用MTT试验测算细胞存活率。取对数生长期鼻咽癌5-8F、SUNE2细胞分为对照组(0μmol/L TP0903)和实验组(TP0903浓度为0.25、0.5μmol/L)。培养24 h后采用划痕试验检测划痕宽度百分比,Transwell小室趋化试验测算穿过Transwell小室的细胞数。采用Transwell小室侵袭试验测算穿过Transwell小室的细胞数。结果鼻咽癌细胞5-8F中,与对照组比较,实验组中1.0、2.0、4.0μmol/L TP0903作用后,细胞存活率均降低,且两两比较,P均<0.05。鼻咽癌细胞SUNE2中,与对照组比较,4.0μmol/L TP0903作用后,细胞存活率降低(P<0.05)。划痕试验结果显示,实验组鼻咽癌细胞的迁移距离较对照组缩短(P均﹤0.05)。Transwell小室趋化试验结果显示,与对照组比较,实验组穿过Transwell小室的细胞数均减少,以0.5μmol/L TP0903处理为著(P均﹤0.05)。Transwell小室侵袭试验结果显示,TP0903处理鼻咽癌细胞后,实验组和对照组相比,实验组穿过Transwell小室的细胞数减少,以0.5μmol/L TP0903处理为著(P均﹤0.05)。结论 AXL激酶抑制剂能抑制鼻咽癌细胞的增殖、迁移及侵袭。Objective To investigate the effects of AXL kinase inhibitor TP0903on the proliferation,migration,and invasion of human nasopharyngeal carcinoma cells.Methods Human nasopharyngeal carcinoma cells(5-8F and SUNE2)were chosen and divided into the control group and experiment group.TP0903was not added to the cells of the control group.In the experiment group,0.5,1.0,2.0,4.0μmol/L TP0903was added into5-8F cells,and0.125,0.25,0.5μmol/L TP0903was added into SUNE2cells.MTT was used to detect cell survival rate after72-hour culture.5-8F and SUNE2cells in the logarithmic growth period were chosen and divided into the control group(0μmol/L TP0903)and experiment group(0.25and0.5μmol/L TP0903).The percentage of the scratch width was detected by Scratch test,and the number of cells going through Transwell chamber was detected and calculated by Transwell chamber chemotaxis and invasion tests.Results In the5-8F cells,the cell survival rate significantly decreased after adding1.0,2.0,4.0μmol/L TP0903in the experiment group as compared with that of the control group(P<0.05).In the SUNE2cells,the cell survival rate significantly decreased after adding4.0μmol/L TP0903in the experiment group as compared with that of the control group(P<0.05).Scratch test showed that the migration distance of nasopharyngeal carcinoma cells in the experiment group decreased(P<0.05).Transwell chamber chemotaxis and invasion tests showed that the cells going through Transwell chamber were less in the experiment group than in the control group,especially after adding0.5μmol/L TP0903in cells of the experiment group(P<0.05).Conclusion AXL kinase inhibitor can inhibit the proliferation,migration,and invasion of nasopharyngeal carcinoma cells.

关 键 词：鼻咽癌 AXL激酶抑制剂 细胞增殖 细胞迁移 细胞侵袭 

分 类 号：R739.6[医药卫生—肿瘤]