YY1基因对J82膀胱尿路上皮癌细胞增殖侵袭能力的影响  

Effect of YY1 on proliferation and invasion of bladder urothelial carcinoma in vitro

作　　者：刘翔[1] 耿和[1] 吴宗林[1] 施华娟[1] 张涛[1] LIU Xiang;GENG He;WU Zonglin;SHI Huajuan;ZHANG Tao(People's Hospital of Putuo District,Shanghai 200060,China)

机构地区：[1]上海市普陀区人民医院泌尿外科,上海200060

出　　处：《实用医学杂志》2018年第22期3675-3679,共5页The Journal of Practical Medicine

基　　金：上海市普陀区人民医院院级课题(编号:2015RM108)

摘　　要：目的观察YY1基因在不同表达水平下对J82膀胱尿路上皮癌细胞增殖侵袭能力的影响。方法以转染YY1的J82细胞设为YY1增强表达组,选用靶向YY1序列的shRNA导入J82细胞设为YY1沉默组,并设定对照组。应用Western blot法检测YY1转染效率。以MTT、Transwell实验检测各组细胞增殖和侵袭能力。结果 Western blotting结果显示增强组的YY1表达高于空白组(P=0.001),空白组YY1蛋白表达水平高于沉默组(P <0.05)。MTT实验结果显示YY1增强表达组在培养24 h、48 h和72 h后的吸光度均高于空白组和YY1沉默组(P <0.05)。24 h后空白组和YY1沉默组的吸光度无明显区别(P>0.05)。48 h和72 h后空白组吸光度明显高于YY1沉默组(P <0.05)。Transwell实验结果显示YY1沉默组的细胞计数明显小于空白组和YY1增强组(P <0.05),空白组和YY1增强组之间无明显差异(P> 0.05)。结论沉默YY1基因后可明显减弱J82膀胱尿路上皮癌细胞的增殖和侵袭能力。以YY1为靶点的RNA干扰技术有望成为针对该肿瘤的基因治疗新方法。Objective To investigate the effect of different expression levels of YY1 gene on the prolifera.tion and invasion of J82 bladder urothelial carcinoma cells.Methods The J82 bladder urothelial carcinoma cells were divided into enhanced expression group transfected with YY1,silent expression group transfected withYY1-shRNA and control group.The expression levels of YY1 were detected by western blotting.The proliferation ability was detected by MTT assay and the invasion ability by Transwell assay.Results YY1 expression level in en-hanced expression group was significantly higher than that in control group(P=0.01),and the level in the control group was significantly higher than that in silent expression group(P<0.05).Proliferation ability of enhanced expression group was significantly higher at 24 h,48 h and 72 h(P<0.05).There was no significant difference in the proliferation ability between the control group and silent expression group at 24 h(P>0.05),but the prolifera.tion ability of the control group was significantly higher than that of silent expression group at 48 h and 72 h(P<0.05).Transwell assay showed that invasion ability of silent expression group was significantly lower than that of other groups(P<0.05),and there was no significant difference in invasion ability between control group and enhanced expression group(P>0.05).Conclusion The shRNA of targeting YY1 gene could obviously weakenthe proliferation and invasion ability of J82 bladder urothelial carcinoma cells in vitro.The suppression of YY1 expression by shRNA may be a promising therapy for bladder urothelial carcinoma.

关 键 词：YY1 膀胱尿路上皮癌 增殖 侵袭 

分 类 号：R737.14[医药卫生—肿瘤]