美洲商陆致中国仓鼠肺细胞染色体畸变实验研究  

Experimental research on chromosomal aberration of Chinese hamster lung cells induced by phytolacca americana

作　　者：于蒙 程泽星[1] YU Meng;CHENG Zexing(The Affiliated Hospital of Yangzhou University, Yangzhou, Jiangsu, 225001)

机构地区：[1]扬州大学附属医院,江苏扬州225001

出　　处：《实用临床医药杂志》2018年第23期1-5,9,共6页Journal of Clinical Medicine in Practice

基　　金：中华人民共和国科技部研发计划项目(204030014)

摘　　要：目的探讨美洲商陆对中国仓鼠肺细胞(CHL)染色体的畸变作用。方法四唑盐(MTT)比色法(吸光度490 nm)测定美洲商陆抑制50%CHL细胞生长的浓度(IC50)。确定IC50为染色体畸变试验CHL细胞暴露于受试物的高剂量组,下设100. 0、40. 0、8. 0、1. 6μg/m L共4个剂量组,按常规方法进行CHL细胞染色体畸变试验。在加入与不加入代谢活化系统的条件下,观察CHL细胞暴露于受试物6、24 h CHL细胞染色体数目及结构的变化,进行染色体畸变分析。结果在加入与不加入代谢活化系统的条件下,CHL细胞暴露于受试物6、24 h,美洲商陆的终浓度在200. 0～1. 6μg/m L,CHL细胞染色体畸变率与溶剂对照组(灭菌水和二甲基亚砜)相比无显著差异(P> 0. 05);与阳性对照组相比,差异有统计学意义(P <0. 05)。结论在本试验条件下,美洲商陆不诱发中国仓鼠肺细胞染色体结构畸变。Objective To explore the role of teratogenicity of phytolacca americana in chromosome of Chinese hamster lung cells(CHL).Methods The MTT assay(OD490nm)was used to measure the concentration of phytolacca americana for inhibiting50%of cell growth(IC50),and IC50was identified as the highest dose when CHL cells exposed to the test substances.In this circumstance,the four groups with doses of100.0,40.0,8.0and1.6μg/mL were set up,and were processed with convention method for chromosome aberration of CHL cell.The differences of number and structural changes of CHL cell chromosome exposed to the test substance after6and24hours with metabolic activation system or not were observed.Results CHL cells were exposed to the tested substances for6and24hours with or without metabolic activation system,and the final concentration of phytolacca americana was in the range of200.0to1.6μg/mL.There was no significant differences between the different concentrations of phytolacca americana and the control group(sterile water and DMSO)(P>0.05),but there were significant differences when compared to positive control group.Conclusion Under the present experimental conditions,phytolacca americana didnot induce chromosomal structural aberration of Chinese hamster lung cells.

关 键 词：美洲商陆 肺细胞 半数抑制浓度 染色体畸变 吸光度 

分 类 号：R563[医药卫生—呼吸系统]