均匀设计法优化香水柠檬果皮绿色素提取工艺  

Study on the extraction process of the green pigment from Citrus Limon(L.) Burm. F. peel by homogeneous design method

作　　者：王兆卫 蔡瑜 周旋 翟晓瑞 霍明洋 高锐 郗艳丽 WANG Zhaowei;CAI Yu;ZHOU Xuan;ZHAI Xiaorui;HUO Mingyang;GAO Rui;XI Yanli(School of Public Health,Jilin Medical University,Jilin City,Jilin Province,132013,China)

机构地区：[1]吉林医药学院公共卫生学院 [2]吉林医药学院公共卫生学院预防医学教研室,吉林吉林132013

出　　处：《吉林医药学院学报》2019年第1期18-21,共4页Journal of Jilin Medical University

基　　金：吉林省科技厅项目资助(20170623093-11TC);吉林省卫生计生委资助项目(2007Q047);吉林省大学生创新创业计划训练项目(201708)

摘　　要：目的探讨均匀设计法优化香水柠檬果皮绿色素提取工艺并观察其稳定性。方法选取市售香水柠檬,利用甲醇、乙醇和水作为提取溶剂,以筛选最佳的提取溶剂。用均匀设计法优化提取方案,提取并测定提取液中的绿色素吸光度,分析不同提取方案的提取结果。对最佳提取方案获得的绿色素进行稳定性探究。结果乙醇的提取效果最佳。最佳提取工艺为提取时间210 min,提取温度30℃,液料比为5∶1(m L/g),乙醇体积分数为90%,在此条件下获得的提取液吸光度均值为(4. 67±0. 37)。山梨酸钾、氯化钠、日光灯照射和阳光照射处理对提取液吸光度影响较大,柠檬酸、维生素C和避光处理对绿色素的吸光度影响不显著。结论该提取工艺能获得含量较高的绿色素,但所提取绿色素的稳定性易受食品添加剂和保存条件的影响,避光保存可避免绿色素降解。Objective To study the extraction process of the green pigment from Citrus Limon(L.)Burm.F.peel by homogeneous design method and its stability.Methods The best extractant of green pigment from Citrus Limon Burm.F.peel was selected from methanol,ethanol and water which was sold in the market.Extraction process were optimized by homogeneous design method.The results of different extraction process were tested by measuring the absorbance of green pigment to optimize the best one.Then the stability of green pigment extracted under the optimal process were analyzed.Results The results showed that ethanol was suitable for extracting green pigment of Citrus Limon(L.)Burm.F.peel and the optimum extractions were 210 min,temperature of 30℃,ratio of liquid to material as 5∶1(mL/g),ethanol concentration of 90%.Under the optimum extraction condition,the average absorbance of green pigment extracted was(4.67±0.37).The absorbance of green pigment extracted was obviously influenced by potassium sorbate,sodium chloride,fluorescent lamp irradiation and sunlight irradiation,while there was no significantly influenced on the stability of green pigment extracted by the citric acid,vitamin C and photophobic treatment.Conclusion The high green pigment yield can be obtained by our optimum extraction process,the stability of the extracted liquid is easily affected by food additives and storage conditions,and the degration can be prevented by keeping it away from light.

关 键 词：香水柠檬 绿色素 均匀设计法 提取工艺 

分 类 号：TS272.3[农业科学—茶叶生产加工]