微小RNA-497抑制甲状腺乳头状癌细胞活力、侵袭和迁移  

MicroRNA-497 inhibits viability,invasion and migration of thyroid papillary cancer cells

作　　者：张莉君[1] 叶颖[1] 张遂亮[1] 庄菊花 夏伟[1] ZHANG Li-jun;YE Ying;ZHANG Sui-liang;ZHUANG Ju-hua;XIA Wei(Seventh People’s Hospital, Shanghai University of TCM, Shanghai 200137, China)

机构地区：[1]上海中医药大学附属第七人民医院,上海200137

出　　处：《中国病理生理杂志》2018年第12期2160-2165,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81703755);上海市浦东新区卫生系统重点学科建设项目(No.PWZxk2017-06);上海中医药大学预算内(自然科学类)项目(No.2016YSN67);上海中医药大学附属第七人民医院人才培养计划(No.XX2017-01)

摘　　要：目的:研究微小RNA-497(miR-497)抑制甲状腺乳头状癌(PTC)细胞活力、侵袭和迁移的作用机制。方法:采用Target Scan 6. 0软件预测miR-497的靶基因,通过萤光素酶报告基因检测法、RT-qPCR和Western blot进行靶基因验证,再通过RT-qPCR检测PTC组织中miR-497及其靶基因的表达,并通过基因转染研究miR-497及其靶基因对PTC细胞活力、侵袭和迁移的影响。结果:Target Scan 6. 0软件预测、萤光素酶报告基因检测法、RTq PCR和Western blot均证明AKT3是miR-497的直接靶基因。此外,PTC组织中的AKT3表达水平较正常组织高(P <0. 05),且与miR-497表达呈负相关(r=-0. 573 7,P <0. 01)。下调AKT3可以抑制PTC细胞的活力、迁移和侵袭,与miR-497过表达在PTC细胞中具有相似的作用。结论:miR-497通过直接靶向调节AKT3抑制甲状腺乳头状癌细胞的活力、侵袭和迁移。AIM:To investigate the underlying mechanisms of mircoRNA-497(miR-497)inhibiting the viability,migration and invasion of papillary thyroid cancer(PTC)cells.METHODS:TargetScan6.0was used to predict the potential targets of miR-497.The target gene was confirmed by luciferase reporter assay,RT-qPCR and Western blot.The expression levels of miR-497and its target gene in the PTC tissues were detected by RT-qPCR.Gene transfection,MTT assay,and cell migration and invasion assays were used to investigate the effects of miR-497and its target gene on PTC cell viability,migration and invasion.RESULTS:AKT3was demonstrated to be the direct target gene of miR-497.In addition,AKT3expression was higher in the PTC tissues than that in normal tissues(P<0.05)and negatively correlated with miR-497expression(r=-0.5737,P<0.01).Furthermore,down-regulation of AKT3also suppressed cell viability,migration and invasion of PTC,which played similar roles of miR-497over-expression in PTC cells.CONCLUSION:miR-497inhibits the viability,migration and invasion of PTC cells by directly targeting AKT3.

关 键 词：微小RNA-497 AKT3基因 甲状腺乳头状癌 

分 类 号：R736.1[医药卫生—肿瘤] R730.23[医药卫生—临床医学]