红三叶根际溶磷菌株分泌有机酸与溶磷能力的相关性研究  

Relationship between organic acids secreted from rhizosphere phosphate-solubilizing bacteria in Trifolium pratense and phosphate-solubilizing ability

作　　者：李海云[1] 姚拓[1] 张榕 张洁 李智燕 荣良燕 路晓雯 杨晓蕾 夏东慧 罗慧琴 LI Hai-yun;YAO Tuo;ZHANG Rong;ZHANG Jie;LI Zhi-yan;RONG Liang-yan;LU Xiao-wen;YANG Xiao-lei;XIA Dong-hui;LUO Hui-qin(College of Pratacultural Science, Gansu Agricultural University, Lanzhou 730070, China;Grassland Station of Gansu Province, Lanzhou 730010, China)

机构地区：[1]甘肃农业大学草业学院,甘肃兰州730070 [2]甘肃省草原技术推广总站,甘肃兰州730010

出　　处：《草业学报》2018年第12期113-121,共9页Acta Prataculturae Sinica

基　　金：甘肃省草业技术创新联盟科技支撑项目(GCLM2016006);甘肃省农业生物技术研究与应用开发项目(GNSW-2016-36)资助

摘　　要：为初步探究红三叶根际溶磷菌株分泌有机酸与溶磷能力的相关性。利用改良PKOC2液体培养基对筛选自红三叶根际的4株优良溶磷菌进行液体培养,采用钼蓝比色法和高效液相色谱法动态监测溶磷菌培养期间发酵液pH、溶磷量及分泌有机酸的种类和数量。结果表明:菌株MHS7、MHS27、MHS30和MHS49均能分泌乳酸、草酸、苹果酸、富马酸和丁二酸,其中菌株MHS30还能够分泌酒石酸;菌株MHS7溶磷量与pH呈负相关(P>0.05);菌株MHS27溶磷量与pH呈正相关(P>0.05),与分泌乳酸量呈显著正相关(P<0.05);菌株MHS30溶磷量与pH呈显著负相关(P<0.05),与分泌有机酸总量呈极显著正相关(P<0.01),与分泌酒石酸呈显著正相关(P<0.05);菌株MHS49溶磷量与分泌丁二酸呈显著正相关(P<0.05);各菌株分泌有机酸的种类和数量差异较大,溶磷菌的溶磷量与总有机酸量存在一定相关性,但有效磷增量并不完全由总有机酸量来决定,有机酸的种类和数量对溶磷量也有一定的影响。不同菌株溶磷能力与其分泌的有机酸种类和含量之间的关系存在多样性,不同溶磷菌株存在不同的溶磷途径。This study represents a preliminary exploration of the relationship between organic acid secretion and the phosphate-dissolving ability of rhizosphere phosphate-solubilizing bacteria.Four strains of excellent phosphate-solubilizing bacteria were selected from Trifolium pratense and cultured in improved PKOC2liquid medium.The pH,phosphate-solubilizing ability,and the types and quantities of organic acids secreted were dynamically monitored by molybdenum blue colorimetry and high performance liquid chromatography(HPLC).The results showed that the strains MHS7,MHS27,MHS30,and MHS49all secreted lactic acid,oxalic acid,malic acid,fumaric acid,and succinic acid,and strain MHS30also secreted tartaric acid.In strain MHS7,the phosphate-solubilizing ability was negatively correlated with pH(P>0.05).In strain MHS27,the phosphate-solubilizing ability was positively correlated with pH(P>0.05)and lactic acid content(P<0.05).In strain MHS30,the phosphate-solubilizing ability was negatively correlated with pH(P<0.05),and positively correlated with the amount of organic acids secreted(P<0.01)and the tartaric acid content(P<0.05).In strain MHS49,the phosphate-solubilizing ability was significantly positively correlated with the succinic acid content(P<0.05).The types and quantities of organic acids secreted differed among the four bacterial strains.The phosphate-solubilizing ability of phosphate-solubilizing bacteria was correlated with the total organic acids content,and was also influenced by the types and quantities of organic acids released.However,the effective increase in effective phosphorus content was not determined by the total organic acids content.There were diverse relationships between the phosphate-solubilizing ability of different bacterial strains and the types and amounts of organic acids they released.These findings suggested that different phosphorus-solubilizing pathways exist in different strains of phosphate-solubilizing bacteria.

关 键 词：溶磷菌 溶磷量 有机酸 相关性 

分 类 号：S154.3[农业科学—土壤学] S541.2[农业科学—农业基础科学]