5-aza-2dC对乳腺癌细胞增殖及PRDM2和PRDM5甲基化的影响  

Impacts of 5-aza-2dC on breast cancer cell proliferation and methylation of PRDM2 and PRDM5

作　　者：白杰[1] 张晓宇 康晓宁[1] 靳丽君 张勤[1] 邵建强 王遵义[1] Bai Jie;Zhang Xiaoyu;Kang Xiaoning;Jin Lijun;Zhang Qin;Shao Jianqiang;Wang Zunyi(SectionⅢof Department of Surgical Oncology,Cangzhou Central Hospital,Cangzhou,Hebei,061001,China;Section Hof Department of Ultrasound,Cangzhou Central Hospital,Cangzhou,Hebei,061001,China)

机构地区：[1]沧州市中心医院肿瘤外三科,河北沧州061001 [2]沧州市中心医院超声二科

出　　处：《西南国防医药》2018年第12期1159-1162,共4页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的探讨5-氮-2'脱氧胞苷(5-aza-2dC)对人乳腺癌MDA-MB-231细胞增殖的影响和对PR区锌指蛋白2(PRDM2)、锌指蛋白5(PRDM5)甲基化及表达水平的影响。方法培养人乳腺癌MDA-MB-231细胞,分别采用0(对照组)、1、2.5、5、10μmol/L 5-aza-2dC作用1、2、3、4、5 d后,采用MTT法检测细胞生长情况,MSP检测PRDM2和PRDM5甲基化水平,实时荧光定量PCR(qRT-PCR)和Western-blot检测PRDM2和PRDM5 mRNA和蛋白表达水平。结果 5-aza-2dC作用后,MDA-MB-231细胞增殖能力显著低于对照组(P <0.05),且随着5-aza-2dC浓度增加,抑制作用逐渐增强,作用5 d时,抑制作用最强;5-aza-2dC作用5 d后,与对照组比较,5-aza-2dC处理组细胞PRDM2和PRDM5甲基化水平均显著降低,mRNA和蛋白相对表达水平均显著增高,呈一定浓度依赖性。结论 5-aza-2dC可浓度依赖地抑制人乳腺癌MDA-MB-231细胞增殖的能力,降低细胞中PRDM2和PRDM5甲基化水平,促进PRDM2和PRDM5表达。Objective To explore the impacts of 5-azote-2’-deoxycytidine(5-aza-2 dC) on the proliferation of human breast cancer cell MDA-MB-231 and the methylation and expression level of PR domain zinc finger protein 2(PRDM2) and domain zinc finger protein 5(PRDM5). Method s Human breast cancer cells MDA-MB-231 were cultured and treated with 5-aza-2 dC of 0(the control group), 1, 2.5, 5 and 10 μmol/L for one, two, three, four and five days respectively. MTT method was used to detect the growth of the cells, MSP was adopted to detect the methylation level of PRDM2 and PRDM5, real-time fluorescence quantification polymerase chain reaction(qRT-PCR), and Western-Blot was adopted to detect the mRNA and protein expression level of PRDM2 and PRDM5.Results After the treatment with 5-aza-2 dC, the proliferation of breast cancer cell MDA-MB-231 was significantly lower than that in the control group(P < 0.05); the inhibition gradually increased with the increase of the concentration of 5-aza-2 dC and reached the highest level after five days of treatment. After five days of treatment, compared with the control group, the methylation level of PRDM2 and PRDM5 in cells treated with 5-aza-2 dC was significantly lower, but relative expression levels of mRNA and protein were significantly higher and showed certain concentration dependence. Conclusion 5-aza-2 dC can inhibit the proliferation of human breast cancer cell MDA-MB-231, lower the methylation level of PRDM2 and PRDM5 and promote the expression of PRDM2 and PRDM5.

关 键 词：5-氮-2'脱氧胞苷 乳腺癌 肿瘤转移 PR区锌指蛋白 甲基化 

分 类 号：R730.5[医药卫生—肿瘤]