Identification of In-intro Antagonistic Activity of HAS1 Protein against Sporisorium scitanminea  

作　　者：Huiqing LIU Yuantao HUANG Mengyu DAI Yimin QIU Gengfeng ZHAO Guoru XIONG 

机构地区：[1]College of Basic Medicine and Life Science,Hainan Medical University [2]Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences

出　　处：《Agricultural Biotechnology》2018年第6期9-11,15,共4页农业生物技术（英文版）

基　　金：Supported by College Students'Innovation and Enterpreneurship Training Program of Hainan Province(2017116);College Students' Innovation and Enterpreneurship Training Program of Hainan Medical University(HYCX2016073);Natural Science Foundation of China(31471555)

摘　　要：Sugarcane smut greatly influences yield and quality of sugarcane.HAS1 gene in biocontrol strain HAS antagonistic to Sporisorium scitanminea was selected for prokaryotic expression vector construction and inducible expression,and the antagonistic effect of HAS1 protein was detected in vitro.The results showed that the expression product of antifungal HAS1 gene obtained through prokaryotic expression vector construction and inducible expression had better antagonistic effect against S.scitanminea.This study provides an experimental basis for further utilization of this protein.Sugarcane smut greatly influences yield and quality of sugarcane.HAS1 gene in biocontrol strain HAS antagonistic to Sporisorium scitanminea was selected for prokaryotic expression vector construction and inducible expression,and the antagonistic effect of HAS1 protein was detected in vitro.The results showed that the expression product of antifungal HAS1 gene obtained through prokaryotic expression vector construction and inducible expression had better antagonistic effect against S.scitanminea.This study provides an experimental basis for further utilization of this protein.

关 键 词：BACILLUS SUBTILIS HAS Sporisorium scitanminea PROKARYOTIC expression ANTIFUNGAL protein 

分 类 号：S476[农业科学—农业昆虫与害虫防治] S435.661[农业科学—植物保护]