长链非编码RNA DRAIC调控miR-223对胃癌细胞迁移和侵袭的影响  

Effects of DRAIC on the migration and invasion of SGC-7901 gastric cancer cell by regulating miR-223

作　　者：邓清[1] 张铁泉 乐问津[1] 黄强[1] 李宓[1] DENG Qing;ZHANG Tiequan;LE Wenjin;HUANG Qiang;LI Mi(Department of Hepatobiliary Surgery,The First Peoples Hospital of Jingzhou,Jingzhou 434000,Hubei,China)

机构地区：[1]荆州市第一人民医院肝胆外科,湖北荆州434000

出　　处：《西部医学》2018年第12期1753-1757,1762,共6页Medical Journal of West China

摘　　要：目的探讨长链非编码DRAIC调控miR-223对胃癌细胞迁移和侵袭的影响及其机制。方法 qPCR检测DRAIC在胃癌组织和细胞株中的表达情况及差异;双荧光素酶报告基因检测DRAIC与miR-223之间的相互作用;流式细胞术检测DRAIC对胃癌细胞细胞周期和凋亡行为的影响;Transwell侵袭实验检测抑制DRAIC后胃癌细胞侵袭行为的变化情况;划痕愈合实验检测抑制DRAIC后胃癌细胞迁移行为的变化情况。结果与正常胃癌组织相比,胃癌组织中DRAIC的表达水平均相对上调,在SGC7901细胞株中DRAIC表达水平最高,差异有统计学意义(P<0.05);双荧光素酶实验证实DRAIC能与miR-223的3’UTR特异性结合,可以调控miR-223的表达与活性;抑制DRAIC表达后胃癌细胞的凋亡行为得到一定程度的促进;抑制DRAIC的表达后可以抑制胃癌细胞的迁移和侵袭能力。结论DRAIC可以调控miR-223的表达,从而影响胃癌细胞的迁移和侵袭行为。Objective To investigate the effect of long-chain non-coding DRAIC on the migration and invasion of gastric cancer cells and its mechanism.Methods qPCR was used to detect the expression and difference of DRAIC in gastric carcinoma and cell lines.Double luciferase reporter gene was used to detect the interaction between DRAIC and miR-223.The effect of DRAIC on cell cycle and apoptosis of gastric cancer cells was detected by flow cytometry.Transwell invasion assay was used to detect the changes of invasion behavior of gastric cancer cells after DRAIC.Scrap healing test was used to detect the changes of migration behavior of gastric cancer cells after DRAIC.Results Compared with normal stomach tissue,the expression level of DRAIC was up-regulated in gastric cancer tissues.The expression level of DRAIC in SGC-7901 cell line was the highest.The difference was statistically significant.Double luciferase assay confirmed that DRAIC could bind to the 3 UTR of miR-223 and could regulate the expression and activity of miR-223.Inhibition of DRAIC expression can promote apoptosis of gastric cancer cells.Inhibition of DRAIC expression can inhibit gastric cancer cell migration and invasion.Conclusion DRAIC can regulate the expression of miR-223 and affect the migration and invasion of gastric cancer cells.

关 键 词：DRAIC 胃癌 miR-223 细胞侵袭 

分 类 号：R735.2[医药卫生—肿瘤]